Evaluation of wound healing properties of Arrabidaea chica Verlot extract |
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Authors: | Jorge Michelle Pedroza Madjarof Cristiana Gois Ruiz Ana Lúcia Tasca Fernandes Alik Teixeira Ferreira Rodrigues Rodney Alexandre de Oliveira Sousa Ilza Maria Foglio Mary Ann de Carvalho João Ernesto |
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Institution: | CPQBA, PO Box 6171, University of Campinas, Campinas, SP, Brazil. |
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Abstract: | ETHNOPHARMACOLOGICAL RELEVANCE: Arrabidaea chica Verlot. (Bignoniaceae), popularly known as Crajiru, has been traditionally used as wound healing agent. AIM OF THE STUDY: Investigate in vitro and in vivo healing properties of Arrabidaea chica leaves extract (AC). MATERIALS AND METHODS: AC was evaluated in vitro in fibroblast growth stimulation (0.25-250 microg/mL) and collagen production stimulation (250 microg/mL) assays. Allantoin (0.25-250 microg/mL) and vitamin C (25 microg/mL) were used as controls respectively. DPPH and Folin-Ciocalteau assays were used for antioxidant evaluation, using trolox (0.25-250 microg/mL) as reference antioxidant. To study wound healing properties in rats, AC (100mg/mL, 200 microL/wound/day) was topically administered during 10 days and wound area was evaluated every day. Allantoin (100mg/mL, 200 microL/wound/day) was used as standard drug. After treatment, wound sites were removed for histopathological analysis and total collagen determination. RESULTS: AC stimulated fibroblast growth in a concentration dependent way (EC50=30 microg/mL), increased in vitro collagen production and demonstrated moderate antioxidant capacity. In vivo, AC reduced wound size in 96%, whereas saline group showed only 36% wound healing. CONCLUSION: AC efficiency seems to involve fibroblast growing stimulus and collagen synthesis both in vitro and in vivo, beyond moderate scavenging activity, corroborating Crajiru folk use. |
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Keywords: | Bignoniaceae Arrabidaea chica Wound healing Collagen Fibroblast Antioxidant capacity |
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