| 二磷酸果糖的纯化研究 |
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| 引用本文： | 莫晓燕,詹谷宇,项昱红. 二磷酸果糖的纯化研究[J]. 中国医药工业杂志, 2001, 32(3): 100-101,114 |
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| 作者姓名： | 莫晓燕 詹谷宇 项昱红 |
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| 作者单位： | 西安交通大学化工学院生物工程研究所, |
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| 摘 要： | 二磷酸果糖反应液经钙盐沉淀、H^ 型阳离子树脂脱钙，于pH 2．0上Amberlite IR-400CI^-型强碱性阴离子交换柱，pH2．0的0．01mol/L NaCI洗杂，以无机磷的洗脱与消失监测除杂效果，在此条件下可获得高纯度产品。
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| 关 键 词： | 二磷酸果糖 纯化 丝状真菌 沉淀反应 药理学 |
| 文章编号： | 1001-8255(2001)03-100-02 |
Study on Purification of Fructose-1,6-Diphosphate |
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| MO Xiao Yan,ZHAN Gu Yu,XIANG Yu Hong. Study on Purification of Fructose-1,6-Diphosphate[J]. , 2001, 32(3): 100-101,114 |
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| Authors: | MO Xiao Yan ZHAN Gu Yu XIANG Yu Hong |
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| Abstract: | Fructose 1,6 diphosphate with high purity was obtained using Amberlite IR 400 Cl - resin from anion exchange chromatography with a solution of pH 2.0 0.01 mol/L NaCl to remove other metabolites of glycolysis and phosphorus from the crude product (dicalcium salt). The process of removing impurities was determined by observing the reduction and disappearance of phosphorus in the eluate. |
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| Keywords: | fructose 1 6 diphosphate purification phosphorus |
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