miR-617在宫颈癌组织中的表达及其对宫颈癌SiHa细胞生物学行为的影响

目的:探讨miR-617在宫颈癌组织中的表达及其过表达对宫颈癌SiHa细胞增殖、凋亡、迁移等细胞生物学行为的影响。方法:采用实时定量PCR法检测宫颈鳞癌组织及正常宫颈组织中miR-617表达。宫颈癌SiHa细胞中转染miR-617模拟物使其过表达,采用CCK-8细胞增殖检测试剂盒检测细胞增殖活力,流式细胞术检测细胞凋亡,平板克隆实验检测细胞克隆形成率,划痕实验检测细胞迁移能力,Western blot法检测靶基因C-末端结合蛋白1(Ct BP1)蛋白表达水平。结果:miR-617在宫颈鳞癌组织中表达下调,过表达miR-617可抑制宫颈癌SiHa细胞增殖,促进SiHa细胞凋亡,抑制SiHa细胞的克隆形成能力,抑制细胞的迁移,过表达miR-617可降低CtBP1蛋白表达。结论:miR-617在宫颈鳞癌中表达下调,可抑制SiHa细胞增殖、促进细胞凋亡、抑制克隆形成和迁移,其作用机制可能与CtBP1蛋白下调有关。

The expression of miR-617 in cervical cancer and the effects on cell biological behaviors ;in cervical carcinoma SiHa cell

Objective:To investigate the expression of miR-617 in cervical cancer and the effects of its overexpression on cell biological behaviors such as proliferation,apoptosis and migration in cervical carcinoma SiHa cell. Methods:The expressions of miR-617 in cervical cancer and normal tissue were detected by real time RT-PCR,and miR-617 mimics were trans-fected into SiHa cell to make it overexpression, cell proliferation activity was determined by CCK-8 cell proliferation assay Kit,and cell apoptosis was detected by flow cytometry,flat cell cloning experiments was used toestimate cell colony-forming ability,cell migration were tested by wounding healing assay,and the expression of target genes c-Terminal binding protein 1 ( Ct-BP1) protein was determined by Western blot. Results:The expression of miR-617 in cervical cancer was down-regulated,and overexpression of miR-617 could inhibit SiHa cell proliferation, and promote cell apoptosis,and could inhibit SiHa cellclone formation as well as cell migration, overexpression of miR-617 downregulated the expression of protein CtBP1 . Conclusion:miR-617 in cervical cancer were down-regulated,and miR-617 can inhibit SiHa cell proliferation, clone formation and migration,and promote cell apoptosis,the mechanism may be relate to the downregulation of protein CtBP1 .