水浸法提取银杏叶总黄酮工艺的研究 |
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引用本文: | 闫高颖,张必荣,张敏,周阳,郭倩茹,杨妍,张勇. 水浸法提取银杏叶总黄酮工艺的研究[J]. 西北药学杂志, 2016, 0(6): 560-562. DOI: 10.3969/j.issn.1004-2407.2016.06.003 |
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作者姓名: | 闫高颖 张必荣 张敏 周阳 郭倩茹 杨妍 张勇 |
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作者单位: | 陕西天谷生物科技集团有限公司,西安,710065 |
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摘 要: | 目的优选水浸法提取银杏黄酮工艺的最佳条件。方法采用正交实验以及高效液相色谱法,对水提银杏总黄酮的条件进行了优选。结果浸提温度、浸提时间、提取次数和料液比对银杏叶总黄酮的提取率均有影响;最佳提取工艺为:100℃条件下水浸法提取3次,每次1h,料液比为1∶26。结论该提取工艺简单可行,稳定,适合大生产。
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关 键 词: | 黄酮 银杏提取物 水浸提取 正交实验 |
Study on the water extraction of total flavones from Ginkgo leaves |
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Abstract: | Objective To study the optimal water extraction conditions of total flavones from Ginkgo leaves.Methods Using orthogo-nal test and HPLC,the water extraction conditions was optimized for total flavones from Ginkgo leaves.Results Extraction rate of total flavones was affected by the extraction temperature,extraction time,extraction times and material-liquid ratio.The optimal extraction conditions were concluded as follows:water as the extractant,material-liquid ratio 1∶26,extraction 3 times (each time 1 h)and extraction temperature 100 ℃.Conclusion The proposed procedure is simple,feasible,stable and suitable for mass produc-tion. |
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Keywords: | flavones Ginkgo biloba extract water extraction orthogonal test |
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