HPLC法测定葛花中大豆苷和染料木苷的含量

目的建立HPLC法测定葛花中大豆苷、染料木苷的含量。方法采用C18色谱柱(250mm×4.6mm,5μm);流动相为乙腈-1.0mL·L-1磷酸溶液(15∶85);流速为1.0mL·min~(-1);检测波长为260nm;柱温为30℃。结果大豆苷回归方程为Y=166 989.8 X-1 438.6,r=0.999 9,大豆苷在0.206~1.032μg范围内线性关系良好;染料木苷回归方程为Y=142 567 X+2 543.1,r=0.999 7,染料木苷在0.204~1.018μg范围内线性关系良好。结论该方法简便、准确,重复性好,可作为葛花的质量评价依据。

Determination of daidzin and genistin glycosides in the flower of kudzuvine by HPLC

Objective To establish an HPLC method for the determination of the content for daidzin,genistin glycosides in the flower of kudzuvine.Methods C1 8 column (250 mm×4.6 mm,5 μm)was used with acetonitrile-1 mL·L-1 phosphoric acid (1 5∶85)as the mobile phase;the flow rate was 1.0 mL·min-1 ,the detection wavelength was 260 nm,and the column temperature was 30 ℃. Results Daidzin regression equation was Y = 1 66 989.8X - 1 438.6,r =0.999 9,indicated that daidzin injection amount between 0.206-1.032 μg had good linear relationship;genistein glycosides regression equation was Y = 142 567X +2 543.1,r =0.999 7, showed that genistein glycosides injection amount between 0.204-1.018 μg had good linear relationship.Conclusion The method is simple,accurate,reproducible,and can be used as a quality assessment method for the flower of kudzuvine.