一测多评法测定天然辣椒碱中2种主要成分

目的建立以1种对照品同时测定天然辣椒碱中2种成分的质量评价方法。方法以辣椒辣素为对照,采用一测多评法,同时测定辣椒辣素和二氢辣椒辣素的含量。结果测得辣椒辣素与二氢辣椒辣素的相对校正因子为1.12,同时采用外标法和一测多评法测定样品中二氢辣椒辣素含量,采用t检验对二者测定值进行比较,二者之间无显著差异(P>0.05);以辣椒辣素的保留时间为1.00,计算得二氢辣椒辣素的相对保留时间为1.355,相对保留时间及保留时间差的RSD均小于5%。结论采用一测多评法,以辣椒辣素为对照,利用相对校正因子可实现同时测定辣椒辣素和二氢辣椒辣素的含量。

Determination of two main ingredients in natural capsaicin extract by quantitative analysis of multi-components with a single-marker

Objective To establish a method for determination of two components in natural capsaicin by one reference substance . Methods Quantitative analysis of multi‐components was performed to determine the contents of dihydrocapsaicin and capsaicin with capsaicin as the reference substance by single‐maker(QAMS) and HPLC .Results The relative correction factor between capsaicin and dihydrocapsaicin was 1 .12 .There was no significant difference of the measured values between the external standard method and QAMS (P>0 .05) .With capsaicin retention time of 1 .00 ,the relative retention time of dihydrocapsaicin was 1 .355 and the RSD was less than 5% .Conclusion With capsaicin as a single marker and the relative correction factor of dihydrocapsai‐cin ,QAMS could be applied for the determination of the main components of natural capsaicin extract simultaneously .