COX2促进卵巢癌细胞迁移及相关机制的研究

目的:探讨COX2对卵巢癌细胞株增殖及迁移的影响及其相关机制。方法:构建慢病毒载体Lenti-COX2-EGFP,转染卵巢癌细胞株SKOV3和ES2。PCR及Western blot法鉴定转染效率,CCK-8实验检测细胞增殖,划痕实验观察细胞迁移能力,qRTPCR法检测E-cadherin、Vimentin、Snail和Slug表达。结果:与对照组相比,过表达COX2后卵巢癌细胞的增殖和迁移能力增强,差异均有统计学意义(P0.05);E-cadherin表达下调,Snail、Slug、Vimentin表达上调。COX2抑制剂塞来昔布处理则可抑制COX2过表达细胞的增殖及迁移能力,与对照组比较差异有统计学意义(P0.05)。结论:COX2可促进卵巢癌细胞增殖及上皮间质转化(EMT)继而增强肿瘤细胞迁移能力;塞来昔布可发挥阻断作用。推测COX2可能是卵巢癌临床治疗的一个潜在靶点。

The effects and related mechanisms cyclooxygenase-2 act in the migration of ovarian cancer cells

Objective:To examine whether COX2 could accelerate proliferation and metastases of ovarian cancer cells and its related mechanisms. Methods:We created Lenti-COX2-EGFP and then transfected ovarian cancer cells. After that, we evaluated the effect of COX2 on proliferation and metastases of ovarian cancer via PCR,Western blot,qRT-PCR,CCK-8 test and healing test. Results:Compare with control group,after up-regulating COX2 expres-sion,cell proliferation and migration accelerated. The expressions of Snail and Slug up-regula-ted,resulting in E-cadherin expression down-regulating and Vimentin expression up-regulating. Celecoxib can inhibit these affections. All of the differences were significant. Conclusion:COX2 can accelerate cell proliferation and EMT,which can accelerate migration. Celecoxib can inhibit this effect. So,COX2 may serve as a potential target for curing ovarian cancer.