钙网蛋白在子宫内膜癌中的表达及临床意义

目的:探讨子宫内膜癌中钙网蛋白(CRT)表达与子宫内膜癌生物学行为的关系。方法:选取2014年2月至2015年1月在中国医科大学附属盛京医院行手术治疗的50例子宫内膜癌组织标本(均为腺癌,术前均未接受放化疗),以及因宫颈CINIII、子宫脱垂等行全子宫切除术的30例正常子宫内膜组织。分别采取实时定量逆转录聚合酶链反应(Real-time PCR)检测CRT mRNA表达,免疫组织化学SP法及蛋白免疫印迹检测CRT蛋白表达水平。分析CRT表达量与子宫内膜癌临床病理学参数的关系。结果:子宫内膜癌组织和正常子宫内膜组织中CRT mRNA相对表达量分别为3.58±3.49和2.00±1.89,CRT蛋白相对表达量分别为0.50±0.19和0.20±0.12,差异均有统计学意义(P0.05)。子宫内膜癌组织中CRT蛋白表达量与TNM分期和有无淋巴结转移有关(P0.05),而与分化程度及有无肌层浸润无关(P=0.129)。结论:CRT蛋白在子宫内膜样腺癌中表达上调,且表达水平与子宫内膜样腺癌的生物学行为可能有一定关系。

Expression and clinical significance of CRT in endometrical carcinoma

Objective:To investigate the expression level and clinicopathological signif-icance of the calreticulin( CRT) in endometrical carcinoma. Methods:From Feb. 2014 to Jan. 2015 ,50 patients with endometrical carcinoma ( all of which were adenocarcinoma without pre-operative chemoradiotherapy ) and 30 cases of normal endometrial tissues, were diagnosed by Shengjing Hospital of China Medical University for surgical treatment. The expression of CRT mRNA in two groups was detected by Real-time PCR,and CRT protein was examined through Western blot and immunohistochemical streptavidin-peroxidase ( SP ) method. And then ana-lyzed the relationship between CRT expression and clinicopathologic parameters. Results:The relative expression of CRT mRNA in endometrial carcinoma and normal endometrial tissue were 3 . 58 ± 3 . 49 and 2 . 00 ± 1 . 89 , and the relative expression of CRT protein in two groups were 0. 50±0. 19 and 0. 20±0. 12. Compared with the normal endometrial tissues,there were signifi-cant differences in the expression of CRT mRNA and protein in endometrial carcinoma tissues ( P<0 . 05 ) . And the expression of CRT protein in endometrial cancer tissues was significantly different in the pathological staging and the lymph node metastasis with statistical significance. Conclusion:The expression of CRT is up-regulated in endometrioid carcinoma,which may play a potential role in the pathogenesis and progression of endometrical carcinoma.