7178株细菌耐药性监测

目的了解2011~2014年我院临床分离菌的分布和耐药特性,为临床合理使用抗菌药物提供参考。方法采用纸片扩散法(K-B法)对我院分离的菌株进行药敏试验并对肠杆菌科细菌进行ESBL检测,按照CLSI 2012年标准判定药敏结果,用WHONET 5.6软件进行数据分析。结果 2011~2014年我院共分离细菌7 178株,其中革兰阴性杆菌5 831株,革兰阳性球菌1 347株。大肠埃希菌和金黄色葡萄球菌分别占首位。大肠埃希菌ESBLs的检出率在55%~66%,肺炎克雷伯菌ESBLs的检出率逐年上升,鲍曼不动杆菌对大部分抗菌药物的耐药率超过60%,铜绿假单胞菌的耐药上升相对平稳,葡萄球菌属MRSA的检出率高于地区平均水平,未检出耐万古霉素菌株。结论我院住院患者分离的细菌耐药性呈逐年上升趋势,应根据药敏检测结果合理使用抗菌药物。

7 178 bacteria drug resistance monitoring

Objective To investigate the distribution and antibiotic resistance profile of clinical isolated bacteria in our hospital during 2011‐2014 .Method Kirby‐Bauer method was used to conduct susceptibility testing and ESBLs detection .The results were ana‐lyzed by WHONET 5 .6 software according to CLSI 2010 .Result A total of 7 178 becteria were isolated from 2011 to 2014 in our hospital in which 5 831 were Gram‐negative bacilli and 1 347 were Gram‐positive cocci .E .coli and Staphylococcus aureus were the most commom pathogens .ESBLs detection rate of E .coli was 55%‐66% .ESBLs detection rate of K lebsiella sp p increased year byyear.TheresistancerateofAcinetobacterspptomostantimicrobialswasmorethan60% .Resistanceofpseudomonasaerugino‐sa raised relatively stable , Staphylococcus MRSA detection rate was higher than the regional average .Vancomycin‐resistant strains were not detected .Conclusion Antimicrobial resistance in clinical bacterial isolates showed a serious situation in our hospi‐tal .Antimicrobial agents should be used rationally based on the results of susceptibility testing .