银杏叶缓释片总黄酮苷释放度测定方法考察

目的：建立测定银杏缓释片中总黄酮苷含量的方法。方法：采用Phenomenex Gemini C18柱（250mm×4．6mm，5μm），以甲醇：0．4％磷酸（65：35）为流动相，检测波长360nm，流速1．0ml·min^-1，柱温30℃。结果：槲皮素、山奈素、并鼠李素的线性范围均为5～50μg·ml^-1，相关系数分别为0．9998、0．9999、0．9996。回收率为99．5％-101．2％。释放度为桨法测定，溶出介质为250ml水，转速为100r·min^-1，12h内呈现良好的释药特征，累积释放量大于95％。结论：本方法简单、准确，为银杏叶缓释片中黄酮类成分的质量控制提供了依据。

Study on Determination of Dissolution Rate of Total Flavonoids in Ginkgo Retard Tablets

Objective： To establish a method for determination of dissolution rate of total flavonoids in ginkgo retard tablets. Method： The total flavonols in ginkgo retard tablets were separated by passing through a Phenomenex Gemini C18 column（250 mm × 4.6 mm, 5 μm） at 30 ℃ using methanol ：0.4% phosphoric acid （ 65 ： 35 ） as mobile phase, flow rate was 1.0 ml·min^-1 An oar method was used to study the dissolution of ginkgo retard tablets, the rotative speed was 100 r·min^-1and the dissolution medium was 250 ml of water. Result： The linear range of quercetin, kaempferide and isorhamnetin was from 5 to 50μg·min^-1, the relevant coefficient of quercetin, kaempferide and isorhamnetin were 0. 999 8,0. 999 9,0.999 6, respectively. The recoveries were between 99.5% - 101.2%. The tablets could continuously release for 12 hours and the dissolution curves in vitro showed that the drug release could be best, the 12 h cumulative drug release rate of microspheres was exceed 95%. Conclusion： The method is simple and accurate, and can be validated as a means offering the basis for the quality control of flavonols in ginkgo retard tablets.