伴放线放线杆菌DNA作为口腔疫苗佐剂的可行性研究

[目的]测定牙周病相关细菌伴放线放线杆菌DNA对口腔内其他细菌性抗原的免疫应答的影响,并探讨牙周病相关细菌伴放线放线杆菌DNA作为口腔疫苗佐剂的可行性.[方法]培养牙周病相关细菌伴放线放线杆菌,并提取其DNA.将大鼠随机分为3个组,采取免疫接种法将等量的明矾缓冲液,明矾-葡萄糖基转移酶缓冲液及含有伴放线放线杆菌DNA的明矾-葡萄糖基转移酶缓冲液分别接种于相应组大鼠体内,分别于接种前及接种后第1,3,6周断尾采血,采用ELISA法测定血清IgG水平.[结果]第1,3,6周时,明矾-葡萄糖基转移酶缓冲液组大鼠血清IgG水平与明矾缓冲液组及含伴放线杆菌DNA明矾-葡萄糖基转移酶缓冲液组相比较均显著增高.[结论]牙周病相关细菌伴放线放线杆菌DNA作为佐剂减弱其他牙周病或龋病细菌抗原的宿主免疫应答,减弱疫苗的效果.

The feasibility study of actinobacillus actinomycetemcomitans DNA as adjuvant of the oral vaccine

OBJECTIVE To detect the effects of DNA from actinobacillus actinomycetemcomitans associated with periodontal disease for the immune response to other bacterial antigens in the oral cavity, and to study the feasibility of DNA from actinobacillus actinomycetemcomitans associated with periodontal disease as adjuvant of oral vaccine. METHODS Actinobacillus actinomycetemcomitans associated with periodontal disease was cultured and its DNA was extracted. 27 of rats were randomly divided into 3 groups, including rats were inoculated with equivalent volume of alum, alum-glucosyl transferase （GTF） buffer and alum-GTF-actinobacillus actinomycetemcomitans DNA by immunifaction, and the blood was collected from the tail in the 0, 1, 3 and 6 weeks, and then the level of serum IgG was detected by ELISA. RESULTS At the first, third and sixth weeks, the level of serum IgG was significantly increased in alum-GTF buffer group as compared with alum group and alum-GTF-actinobacillus actinomycetemcomitans DNA group. CONCLUSION The actinobacillus actinomyeetemcomitans DNA associated with periodontal disease as adjuvant could attenuate the other periodontal disease or host immune response of dental caries relevant antigens and effects of vaccine.