Preliminary proteomic analysis of human serum from patients with laryngeal carcinoma |
| |
Authors: | Hua?Zhang Email author" target="_blank">Xicheng?SongEmail author Xuexia?Liu Qingquan?Zhang Yan?Sun Xiumei?Chen Chenglu?Liu Yan?Wang |
| |
Institution: | (1) Department of Otorhinolaryngology Head and Neck Surgery, Yantai Yu-huang-ding Hospital, No. 20, Yu-huang-ding Dong Road, Yantai, 264000, People’s Republic of China; |
| |
Abstract: | To better evaluate the serum protein alterations in patients with laryngeal carcinoma during surgical treatment process, a
comparative proteomic analysis of human serum from patients with laryngeal carcinoma between pre- and post-operation group
was performed using two-dimensional gel electrophoresis, in combination with matrix-assisted laser desorption ionization time-of-flight
mass spectrometry and database searching. Statistical analysis indicated that 16 gel spots corresponding to 12 proteins altered
their expression significantly between the two groups of patients with laryngeal carcinoma. Of these 12 proteins, 6 proteins
were up-regulated in the pre-operation group. The subsequent Western blot confirmed the results of proteome analysis. Measurement
of MDA and SOD levels in serum combining with bioinformatics analysis indicated the potential roles of the oxidant stress
and immune response as target in monitoring and treating laryngeal carcinoma. The study provides new insight into the laryngeal
carcinoma development and treatment, and the identified proteins are warranted to be further studied. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|