| 槲皮素对人骨肉瘤细胞U-2OS/MTX300增殖和凋亡的影响及其机制研究 |
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| 引用本文： | 尹军强,谢显彪,贾强,王晋,黄纲,邹昌业,沈靖南.槲皮素对人骨肉瘤细胞U-2OS/MTX300增殖和凋亡的影响及其机制研究[J].中国中药杂志,2012,37(5):611-614. |
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| 作者姓名： | 尹军强 谢显彪 贾强 王晋 黄纲 邹昌业 沈靖南 |
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| 作者单位： | 1. 中山大学附属第一医院骨肿瘤科,广东广州,510080
2. 贵州省生物研究所,贵州贵阳,550009 |
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| 基金项目： | 广东省科技计划项目(2007B031405008) |
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| 摘 要： | 目的:研究槲皮素(quercetin,Qu)对骨肉瘤细胞系U-2OS/MTX300增殖和凋亡的作用及其机制。方法:MTT法观察细胞增殖活性;Annexin V/PI染色检测凋亡;线粒体膜电位及细胞色素C的Western blot检测线粒体凋亡途径;持续活化Akt瞬时转染、Western blot检测Akt通路相关蛋白表达水平变化。结果:槲皮素可明显抑制耐甲氨蝶呤骨肉瘤细胞U-2OS/MTX300生长,并呈时间和剂量依赖性;Annexin V/PI可明显检测到细胞凋亡;进一步发现其作用机制是通过下调线粒体膜电位,促进细胞色素C向胞浆释放及抑制Akt磷酸化来实现。结论:槲皮素可显著抑制耐甲氨蝶呤骨肉瘤细胞系U-2OS/MTX300细胞增殖并诱导其凋亡,其机制与线粒体凋亡途径及抑制Akt活性有关。
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| 关 键 词： | 槲皮素 U-2OS/MTX300细胞 凋亡 |
| 收稿时间： | 5/8/2011 12:00:00 AM |
Effect and mechanism of quercetin on proliferation and apoptosis of human osteosarcoma cell U-2OS/MTX300 |
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| YIN Junqiang,XIE Xianbiao,JIA Qiang,WANG Jin,HUANG Gang,ZOU Changye and SHEN Jingnan.Effect and mechanism of quercetin on proliferation and apoptosis of human osteosarcoma cell U-2OS/MTX300[J].China Journal of Chinese Materia Medica,2012,37(5):611-614. |
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| Authors: | YIN Junqiang XIE Xianbiao JIA Qiang WANG Jin HUANG Gang ZOU Changye and SHEN Jingnan |
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| Institution: | Department of Orthopaedic Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Department of Orthopaedic Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, China;Department of Orthopaedic Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Department of Orthopaedic Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Department of Orthopaedic Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Department of Orthopaedic Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China |
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| Abstract: | Objective: To study the effect and mechanisms of quercetin(Qu) on proliferation and apoptosis of human methotrexate resistant osteosarcoma cell U-2OS/MTX300. Method: MTT assay was used to observe cell proliferation. The apoptosis was examined by using Annexin V/PI staining. Western blot of mitochondrial membrane potential and cytochrome c were used to detect mitochondria spoptosis pathway. The protein expressions related to Akt pathway was detected by continuous activated Akt transient transfection and western blot. Result: Qu can obviously inhibit the growth of human MTX resistant osteosarcoma cell U-2OS/MTX300 cells in a dose- and time-dependent manner.Annexin V/PI staining showed obvious cell apoptosis. Reduction of mitochondrial membrane potential, release of mitochondrial cytochrome c to cytosol and dephoshorylation of Akt were observed after Qu treatment. Conclusion: Qu can inhibit proliferation and induce apoptosis of human MTX resistant osteosarcoma cell U-2OS/MTX300, which may be related with mitochondrial apoptosis pathway and Akt activity. |
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| Keywords: | quercetin U-2OS/MTX300 cells apoptosis |
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