SREBP2对口腔鳞癌细胞增殖和迁移的影响及其调控机制

目的： 探讨胆固醇调节元件结合蛋白2（SREBP2）在口腔鳞癌（OSCC）中的调控机制,通过敲低和过表达SREBP2,分析其对口腔鳞癌细胞CAL27增殖、迁移、侵袭的影响及作用机制。方法： 采用实时定量反转录PCR（qRT-PCR）检测SREBP2在OSCC中的表达量。构建siRNA和过表达质粒并转染 CAL27细胞,通过细胞计数（CCK-8）实验和EdU染色检测细胞增殖能力,Annexin V-FITC/PI双染实验检测细胞凋亡水平,划痕愈合和Transwell实验检测细胞迁移、侵袭能力,通过蛋白免疫印迹法检测SREBP2调控MVA通路相关蛋白表达的改变。数据使用Graphpad 5.0软件绘图,组间比较采用t检验。结果： SREBP2在50对口腔鳞癌组织和OSCC细胞系中表达较正常对照组显著降低。敲低及过表达实验表明,SREBP2表达的变化影响OSCC细胞的增殖、迁移、侵袭,并通过MVA信号通路影响OSCC的发展。结论： SREBP2在OSCC中抑制癌细胞增殖、迁移、侵袭并促进凋亡。

Effect of SREBP2 on proliferation and migration of oral squamous cell carcinoma and its regulatory mechanism

PURPOSE: To investigate the effect of sterol regulatory element-binding protein 2 (SREBP2) on the malignant biological behaviors of oral squamous cell carcinoma (OSCC) cell lines. METHODS: The expression of SREBP2 in OSCC was detected by qRT-PCR. SiRNA and overexpression plasmids were constructed and transferred into CAL27. The ability of cell proliferation was detected by cell counting kit-8(CCK-8) assay and EdU cell-proliferation assay; the level of apoptosis was detected by flow cytometry; the ability of migration and invasion was detected by wound-healing assay and Transwell migration and invasion assays. Western blotting was used to detect the expression of SREBP2-related proteins. The data were plotted by Graphpad 5.0; Student's t tests were used to evaluate difference among groups. RESULTS: The expression of SREBP2 in 50 pairs of OSCC tissues and OSCC cell lines was significantly lower than that in normal controls. Knock-down and overexpression experiments showed that the change of SREBP2 expression affected the malignant biological behavior of OSCC cells and affected the development of OSCC through MVA signal pathway. CONCLUSIONS: SREBP2 inhibits proliferation, migration, invasion and promotes apoptosis of cancer cells in OSCC.