纤维蛋白凝胶对上颌扩弓成骨矿化的影响

目的:研究原位注射纤维蛋白凝胶对SD大鼠上颌扩弓成骨矿化的影响.方法:体外实验中,将大鼠骨髓间充质细胞(rBMSCs)在不同凝血酶浓度制备的纤维蛋白凝胶环境下进行共培养,通过CCK-8、ALP染色和活性定量以及茜素红染色,检测梯度浓度凝血酶制备的纤维蛋白凝胶降解产物对rBMSCs细胞增殖和成骨分化的影响.将rBMSCs...

Effect of fibrin glue on osteogenesis and mineralization during rapid maxillary expansion

PURPOSE: To investigate the effect of accelerating osteogenesis and mineralization during rapid maxillary expansion by in situ injection of fibrin glue. METHODS: In in vitro experiments, rat bone marrow stromal cells (rBMSCs) were co-cultured with fibrin glue fabricated with different concentrations of thrombin. The effects of degradation products of fibrin glue on the proliferation and osteogenic differentiation of rBMSCs were tested by CCK-8, alkaline phosphatase (ALP) staining and ALP activity quantification as well as alizarin red staining. rBMSCs were combined with fibrin glue by co-culture, two-dimensional surface culture and three-dimensional culture, respectively. Cell morphology and adhesion were observed by FITC-phalloidine fluorescence staining. In in vivo experiments, 12 6-week male SD rats were used to establish rapid maxillary expansion (RME) model with 100 g expanding force for 7 days. After 7-day expansion, they were divided into two groups: retention for 14 days as control group (R group) while in situ injection of fibrin glue combined with retention for 14 days as experimental group (R+FG group). Micro-CT scanning for three-dimensional reconstruction and analysis were conducted to compare the difference of new bone volume and bone mineral density. The osteogenesis and angiogenesis in mid-palatal suture were observed by H-E staining, Masson's trichrome staining, TRAP staining and sequential fluorescent labeling. GraphPad Prism 8.0.1 software package was used for statistical analysis. RESULTS: The degradation products of fibrin gel had little effect on cell proliferation and osteogenic differentiation of rBMSCs(P>0.05). In addition, rBMSCs cultured in three ways had great cell tractility. Compared with R group, the relative bone volume(P<0.001) and bone mineral density of trabeculae (P<0.001) were significantly increased in R+FG group. A large number of new trabeculae with higher mineralization degree were observed and the number of osteoclasts was decreased in mid-palatal suture. Mineral apposition rate was significantly accelerated in R+FG group(P<0.001). CONCLUSIONS: The degradation products of fibrin glue had no effect on proliferation and differentiation of rBMSCs. Fibrin glue could promote new bone formation, mineralized rate and inhibit osteo-clastogenesis in mid-palatal suture after RME in SD rats, which might be related to regulation of osteogenic differentiation by the surface structure of fibrin glue.