肿瘤坏死因子α和一氧化氮合酶在脓毒症大鼠心功能中的作用初探

目的初步探讨肿瘤坏死因子α（TNF-α）和一氧化氮合酶（NOS）在脓毒症大鼠心功能中的作用。方法制作脓毒症大鼠模型,监测血流动力学,测定大鼠心肌组织中TNF-α和一氧化氮（NO）浓度,检测各型NOS的活性。结果大鼠心肌组织中TNF-α含量2h时达高峰,心肌组织NO 6h时达高峰,与心功能改变时间一致。给予脂多糖（LPS）6h后心脏中cNOS的表达减少;iNOS表达量明显增加。结论脓毒症时,TNF-α和NO发挥重要作用,并且心肌细胞上iNOS表达升高、cNOS表达减少可能在心功能降低中发挥作用。

Role of tumor necrosis factor-alpha and nitric oxide synthetase in cardiac function of rats with sepsis

Objective To study the role of tumor necrosis factor-alpha（TNF-α） and nitric oxide synthetase（NOS） in cardiac function of rats with sepsis.Methods A sepsis model of rats was established.The hemodynamics was monitored,the levels of TNF-α and nitric oxide（NO） were measured,and the activity of NOS was assayed.Results The levels of TNF-α and NO in cardiac muscle tissue reached their peaks 2h after the model was established,which were in accordance with the time of impaired cardiac function.The expression of cNOS was decreased while the expression of iNOS was significantly increased in cardiac muscle cells 6h after the model was established.Conclusion TNF-α and NO play an important role in cardiac function of rats with sepsis.The decreased expression of iNOS and the increased expression of cNOS in cardiac muscle cells may also contribute to the hypofunction of heart in rats with sepsis.