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| 一种适合膜片钳试验的新生大鼠背根神经节细胞的培养方法 | |
| 引用本文： | 马涛,冯泽国,张宏,王维,陶颖. 一种适合膜片钳试验的新生大鼠背根神经节细胞的培养方法[J]. 军医进修学院学报, 2011, 32(5): 501-503 |
| 作者姓名： | 马涛 冯泽国 张宏 王维 陶颖 |
| 作者单位： | 1. 解放军总医院,麻醉手术中心,北京,100853;第二炮兵总医院,麻醉科,北京,100088 2. 解放军总医院,麻醉手术中心,北京,100853 3. 第二炮兵总医院,麻醉科,北京,100088 4. 海军总医院保健科,北京,100037 |
| 摘 要： | 目的建立一种适合膜片钳实验的大鼠乳鼠背根神经节细胞的培养方法。方法在显微镜及显微外科手术器械的辅助下,分离并培养新生大鼠的背根神经元（Dorsal Root Ganglion,DRG）,观察培养细胞的形态,膜片钳记录细胞静息电位水平。结果 DRG神经元细胞形态结构完整,静息电位水平正常,可记录到动作电位及钠电流。结论培养的新生大鼠DRG细胞适合膜片钳试验。 |
| 关 键 词： | 背根神经节 细胞培养 膜片钳 |
A culture method of dosal root ganglion cells from new-born rats in patch clamp experiments |
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| MA Tao,FENG Ze-guo,ZHANG Hong,WANG Wei,TAO Ying. A culture method of dosal root ganglion cells from new-born rats in patch clamp experiments[J]. Academic Journal of Pla Postgraduate Medical School, 2011, 32(5): 501-503 | |
| Authors: | MA Tao FENG Ze-guo ZHANG Hong WANG Wei TAO Ying |
| Affiliation: | MA Tao1,2,FENG Ze-guo1,ZHANG Hong1,WANG Wei2,TAO Ying3 1Anesthesia and Operation Center,Chinese PLA General Hospital,Beijing 100853,China,2Department of Anesthesiology,General Hospital of Second Artillery Forces,Beijing 100088,3Department of Health,Navy General Hospital,Beijing 100037 |
| Abstract: | Objective To establish a culture method of dorsal root ganglion(DRG) cells from rats in patch clamp experiments.Methods DRG cells from rats were isolated using microsurgical instruments under microscope and cultured.Morphology of the cultured cells was observed and their resting electric potential was recorded using patch-clamps.Results The morphological structure of cultured DRG cells was intact,their resting electric potential was normal,and the Na+ channel current could be recorded.Conclusion Cultured DR... |
| Keywords: | Dorsal Root Ganglia Cell Culture Patch-Clamp |
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