| HPLC法测定奈米非肽中三氟醋酸含量 |
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| 引用本文： | 陈宜鸿,李秀敏. HPLC法测定奈米非肽中三氟醋酸含量[J]. 军医进修学院学报, 2011, 32(4): 349-350 |
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| 作者姓名： | 陈宜鸿 李秀敏 |
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| 作者单位： | 1. 解放军总医院医学保障部,北京,100853
2. 北京军区总医院药剂科,北京,100700 |
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| 摘 要： | 目的建立HPLC测定奈米非肽中三氟醋酸含量的方法。方法色谱柱：Apollo C18（4.6mm×250mm,5μm）;流动相：流动相A 0.7%磷酸水溶液（PH3.0）,流动相B甲醇;流速：1.2ml/min;检测波长：210nm;进样量：10μl。结果在12.0-961.2μg/ml浓度范围内,呈良好线性关系,线性方程为A=0.232 2X-0.062 6,r=0.999 6,平均回收率100.7%,RSD为0.73%（n=6）。结论本法简单可靠,重现性好,可作为奈米非肽中三氟醋酸含量测定方法。
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| 关 键 词： | 奈米非肽 三氟醋酸 HPLC 测定 |
Determination of trifluoroactic acid level in nemifitide by HPLC |
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| CHEN Yi-hong,LI Xiu-min. Determination of trifluoroactic acid level in nemifitide by HPLC[J]. Academic Journal of Pla Postgraduate Medical School, 2011, 32(4): 349-350 |
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| Authors: | CHEN Yi-hong LI Xiu-min |
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| Affiliation: | CHEN Yi-hong1,LI Xiu-min2 1Division of Medical Security,Chinese PLA General Hospital,Beijing 100853,China,2Department of Pharmacy,Beijing Army General Hospital,Beijing 100700 |
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| Abstract: | Objective To establish the HPLC method for measurement of trifluoroactic acid level in nemifitide.Methods Chromatogram with an Apollo C18 column（4.6mm×250mm,5μm）,mobile phase with a 0.7% phosphoric acid aqueous solution（pH3.0）,methanol with a flow velocity of 1.2ml/min,test wavelength at 210nm,and an injection dose of 10μl,were used to establish the HPLC method for measurement of trifluoroactic acid level in nemifitide.Results The method had a good linear relation when the regression equation was A=0.232 2X-0.062 6 and r=0.999 6 at the concentration of 12.02-961.2μg/ml.The average recovery rate was 100.7% and the RSD was 0.73%（n=6）.Conclusion The method is simple and reliable with a good repeatability,and can thus be used in measurement of trifluoroactic acid level in nemifitide. |
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| Keywords: | HPLC |
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