| STEAP4基因在人前体脂肪细胞诱导分化过程中的表达 |
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| 引用本文： | 陈小慧,赵亚萍,高春林,张春梅,朱春,朱金改,郭锡熔.STEAP4基因在人前体脂肪细胞诱导分化过程中的表达[J].实用儿科临床杂志,2010(7). |
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| 作者姓名： | 陈小慧 赵亚萍 高春林 张春梅 朱春 朱金改 郭锡熔 |
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| 作者单位： | 南京医科大学附属南京妇幼保健院儿科;南京医科大学儿科医学研究所;中国人民解放军第82医院内分泌科; |
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| 基金项目： | 国家自然科学基金(30772364); 教育部基金(20070312001); 江苏省自然科学基金(BK2007230); 南京医科大学基金(07NMUZ024,08NMU-M066) |
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| 摘 要： | 目的观察人肥胖相关全长新基因STEAP4在人前体脂肪细胞诱导分化过程中不同时段表达水平的变化,探讨STEAP4基因与脂肪细胞分化、脂质积聚的关系。方法体外培养人前体脂肪细胞,待细胞生长融合后以1-甲基-3-异丁基黄嘌呤、地塞米松、胰岛素及罗格列酮联合诱导方案,在体外诱导其分化为成熟脂肪细胞。在前体脂肪细胞向成熟脂肪细胞分化过程中观察细胞形态及脂质积聚变化;采用实时荧光定量RT-PCR技术检测诱导分化不同时段(前体,第0、4、6、8、11、14、17天)脂肪细胞中STEAP4基因mRNA的表达水平。结果STEAP4基因高表达于人前体脂肪细胞;加入脂肪细胞分化诱导剂后(第4天)表达略有上调,其后随脂肪细胞分化成熟该基因表达量呈逐渐下降趋势;至脂肪细胞完全分化成熟(第14-17天)表达量最低。STEAP4基因表达水平除在诱导分化前至第4天、第14-17天差异无统计学意义外,余各时间点的表达水平差异均有统计学意义(Pa<0.05)。结论STEAP4基因随脂肪细胞分化成熟表达逐渐下调,可促进脂肪细胞的分化成熟和脂质积聚,与肥胖的发生有关。
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| 关 键 词： | STEAP4基因 人前体脂肪细胞 分化 |
Expression of STEAP4 Gene during the Period of Human Preadipocyte Differentiation |
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| CHEN Xiao-hui,ZHAO Ya-ping,GAO Chun-lin,ZHANG Chun-mei,ZHU Chun,ZHU Jin-gai,GUO Xi-rong.Expression of STEAP4 Gene during the Period of Human Preadipocyte Differentiation[J].Journal of Applied Clinical Pediatrics,2010(7). |
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| Authors: | CHEN Xiao-hui ZHAO Ya-ping GAO Chun-lin ZHANG Chun-mei ZHU Chun ZHU Jin-gai GUO Xi-rong |
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| Institution: | CHEN Xiao-hui1,2,ZHAO Ya-ping2,3,GAO Chun-lin1,ZHANG Chun-mei1,ZHU Chun1,ZHU Jin-gai1,GUO Xi-rong1,2 (1.Department of Pediatrics,Nanjing Maternal , Child Health Hospital Affiliated to Nanjing Medical University,Nanjing 210004,Jiangsu Province,China,2.Institute of Pediatrics Medicine,Nanjing Medical University,Nanjing 210029,3.Department of Endocrinology,the 82nd Hospital of the Chinese People\'s Liberation Army,Huai\'an 223001,China) |
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| Abstract: | Objective To observe the expression of STEAP4 gene(a novel obesity-related gene) during the period of human preadipocyte differentiation and to explore the relationship between the STEAP4 gene expression and adipocytes differentiation,adipogenesis.Methods Human preadipocytes were cultured and differentiated into the matured adipocytes in vitro.Adipocytes morphology and lipid accumulation were observed during this process.Total RNA was extracted from adipocytes at various time points (preadipocyte,Day 0,Day ... |
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| Keywords: | STEAP4 gene human preadipocyte differentiation |
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