巢式聚合酶链反应检测慢性粒细胞白血病bcr／abl融合基因

目的 探讨Ph染色体和bcr/abl融合基因在慢性粒细胞白血病（CGL）的发病机制、诊断、治疗、预后判断的价值。方法 对46例CGL患者作巢式逆转录聚合酶链反应（RT-PCR）检测bcr/abl融合基因，同时对其中28例作细胞遗传学检查。结果 46例CGL患者中，44例bcr/abl融合基因阳性，阳性率为95.7%;28例CGL患者作细胞遗传学检查。26例Ph染色体阳性，阳性率为92.9%；2例Ph染色体阴性的CGL患者，用RT-PCR检测出ber/abl融合基因。结论 巢式RT-PCR是一种快速、敏感而准确的检测方法，可以为部分Ph染色体阴性的CGL患者提供分子生物学的诊断依据。

Detection of bcr/abl fusion gene in chronic granulocytic leukemia by nested reverse transcriptase polymerase chain reaction

Objective To evaluate the value of the Philadelphia chromosome and bcr/abl fusion gene in the pathogenesis, diagnosis, treatment and prognosis of chronic granulocytic leukemia (CGL). Methods Using bcr/abl fusion genes were detected by nested reverse in 46 CGL patients. The Philadelphia chromosome were detected by cytogenetic method in 28 of these patients.Results The positive rate of bcr/abl fusion gene was 95.7% .The positive rate of Philadelphia was 92.9%.The bcr/abl fusion genes were found in patients with negative Philadelphia chromosome. Conclusion Nested RT PCR is a rapid, sensitive and precise method for bcr/abl fusion gene in CGL, providing molecular biological basis for diagnosis of CGL with negative Philadelphia chromosome.