清热养阴药作用后的脐血细胞浆逆转白血病细胞表型研究

在对急性白血病(AL)中医发病机制探讨基础上，认为清热养阴中药可以对抗AL细胞形成的基础。让人脐血造血细胞在清热养阴药作用下发育，建造与AL细胞表型相反的细胞，再以其胞浆逆转AL细胞表型。实验结果表明：清热养阴药能使造血细胞增殖能力下降而分化能力加强，造血调控因子发生重新调整与组合；中药作用后的脐血细胞浆能够逆转HL60细胞表型，表现为向成熟单核巨噬细胞分化，分裂能力下降，增殖抑制，出现凋亡。

The Phenotype Reversion of Human Myelogenous Leukemic Cells Caused by Human Cord Blood Cytoplasm Treated with TCM Drugs for Clearing Away Heat and Nourishing Yin

In this experiment the human cord blood hematopoietic cell (HCBHC) was used to cultivate a kind of cell whose phenotype is contrary to that of the cell with acute leukemia by a TCM drug culture method. First, HCBHC was cultivated with TCM drugs for clearing away heat and nourishing yin (CHNY), and then, its cytoplasm was used to convert the phenotype of the cell with acute leukemia. The results showed that TCM drugs for CHNY, could decrease HCBHC multiplication rate, increase HCBHC differentiation rate, and cause the readjustment and recombination of HCBHC hematopoietic modulators; and the phenotype of HL60 cells could be converted by HCBHC cytoplasm cultivated with TCM drugs for CHNY, manifesting mature mononuclear macrophage differentiation, declined division rate, inhibition of multiplication, and apoptosis.