胶束电动色分离测定山梨酸钾、苯甲酸钠和糖精钠

建立了一个胶束电动色谱-二极管阵列检测方法,用于分离测定山梨酸钾、苯甲酸钠和糖精钠.在优化条件下,使用24 mmol/L十二烷基硫酸钠、20 mmol/L硼砂,pH 9.6、电压12 kV,分离时间仅用4.2 min.测定范围内,峰高与分析物浓度呈良好的线性关系,迁移时间和峰高的相对标准偏差(n=6)小于0.63％,3...

Micellar electrokinetic chromatographic separation and determination of potassium sorbate,sodium benzoate and sodium saccharin

A new micellar electrokinetic chromatography with photo diode array detection method was developed to separate and determine potassium sorbate, sodium benzoate and sodium saccharin. In conditions of sodium dodecyl sulphate 24 mmol/L, Na2B4O7 20 mmol/L, pH 9.6, and separation voltage 12 kV, the separation was achieved within 4.2 min. Good linear relationships between peak height and concentration were found in the tested range. Relative standard deviations （n=6） of migration times or peak heights were less than 0.63%. The detection limits for potassium sorbate, sodium benzoate and sodium saccharin were 0.42, 0.43, and 0.42 μg/L respectively. The sensitivity was improved ten to a hundred times more than those of high performance liquid chromatography method reported recently. The method was applied to the determination of the three food additives in real drink samples with recoveries of 89.3%-112.0%.