阿霉素诱导大鼠心衰模型的建立

目的:通过阿霉素(Adriamyc in,ADR)腹腔注射给药(Adriamyc in,ADR)试图造成大鼠心衰,探讨建立大鼠阿霉素心衰动物模型的给药方案,为研究心衰的发病机制和有效治疗措施提供理想的动物模型。方法:雄性W istar大鼠25只,随机分成模型组(n=15)和对照组(n=10)。模型组:腹腔注射ADR(4 mg/kg,用注射用水配制成2 mg/m l溶液,即2 m l/kg,每周1次,共6周,累积总量24 mg/kg;对照组:注射相同体积的注射用水。于末次注射停药后2周,测量体重(BW)、心室质量(VW)、动脉收缩压(ASP)、动脉舒张压(ADP)、左室收缩压(LVSP)、左室舒张末压(LVEDP)及左室最大压力上升及下降速度(±dp/dtm ax),并作病理切片观察其组织学变化。结果:与对照组相比,模型组ASP、LVSP、±dp/dtm ax均明显减小,LVEDP明显升高;病理学结果证实符合心肌病样改变。结论:多次间断腹腔注射一定剂量的阿霉素可明显损害心脏的功能,是建立大鼠慢性充血性心力衰竭模型的一种简便可靠的方法。

Establishment of heart failure model induced by adriamycin administration in rats

Objective To study the feasibility of establishment of heart failure model induced by different Adriamycin(ADR) administration in rats,provide a valuable model for the research of pathogenesis and effective therapy in heart failure.Methods Heart failure was induced by intraperitoneal(IP) injection of ADR into male Wistar rats.There were 25 male Wistar rats randomly divided into two groups: The model group was IP injection of ADR at a dose of 4 mg/kg once a week for 6 weeks,the control group received an equivalent volume of sterile water.Two weeks after ADR administration,heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),dp/dt_(max),arterial systolic pressure(ASP) and arterial diastolic pressure(ADP) was detected.Body weight(BW) and ventricular weight(VW) was measured.HE staining and microscopy was used to observe the pathological change in tissue dissection.Results Compared with the control group,ASP,LVSP and dp/dt_(max) of the model group was decreased,VW/BW and LVEDP increased.Pathological examination demonstrated that the cardiac muscular tissue in the model group had characteristics consistent with cardiomyopathy.Conclusion Interval administration of IP injection of ADR in rats injuried systolic and diastolic function of hearts;it is a simple and reliable method to establish a model of chronic congestive heart failure.