MCP-1在SAP大鼠胰腺及肠黏膜组织中的表达

目的 观察单核细胞趋化蛋白-1 （MCP-1）在重症急性胰腺炎（severe acute pancreastitis，SAP）大鼠血清、胰腺及肠黏膜组织中的表达并探讨其机理。方法 24只SD大鼠随机均分为对照组和SAP组。对照组大鼠开腹后仅翻动肠管，SAP组以3%牛磺胆酸钠按0.05 mL/100 g逆行注入大鼠胆胰管（注射时间1 min） 复制SAP模型。2组大鼠分别于制模后12及24 h采集血标本和胰腺及回肠组织标本，进行如下指标检测：①ELISA法测定血清中MCP-1及IL-10水平；②比色法测定淀粉酶（amylase，AMY）水平及二胺氧化酶（diamine oxydase，DAO）水平；③RT-PCR法检测胰腺及回肠组织中MCP-1 mRNA的表达；④观察胰腺及回肠组织的病理学改变。结果 SAP组血清中MCP-1、IL-10、AMY和DAO水平均较对照组升高（P＜0.01）；SAP组胰腺及回肠组织中MCP-1 mRNA表达较对照组上调（P＜0.01），胰腺组织的病理学评分明显增高（P＜0.01）。结论 MCP-1在SAP大鼠血清、胰腺及回肠组织中的表达均上调，且加重了胰腺和肠道组织的损伤。

Expressions of MCP-1 in Pancreatic Tissues and Intestinal Mucosa of Severe Acute Pancreatitic Rats

Objective To observe the effects and mechanism of MCP-1 in ileum and pancreatic tissues in rats with severe acute pancreatitis （SAP）. Methods Twenty-fourth healthy SD rats were randomly divided into two groups： control group （n=12） and SAP model group （n=12）. SAP was induced in model group by retrograde injection of 3% sodium taucrocholate into the biliopancreatic duct of rats. The control group underwent laparotomy with the manipulation of the intestinal canal. The rats were killed at 12 h and 24 h respectively after operation， blood and tissue samples were collected to detect the indexes as follows：① Expressions of MCP-1 mRNA of pancreatic and ileum tissues were detected by RT-PCR；② blood plasma MCP-1 and IL-10 levels were detected by ELISA；③ blood plasma AMY and DAO levels were detected by colorimetry；④ the pathological changes of pancreas and ileum tissues were observed. Results Compared with the control group， the levels of MCP-1， IL-10， AMY， and DAO in plasma， pancreas， and ileum tissues were signi- ficantly increased in SAP model group （P＜0.01）， the expressions of MCP-1 mRNA in pancreas and ileum tissues were up-regulated simultaneously （P＜0.01）， and pathological scoring increased obviously （P＜0.01）. Conclusion The levels of MCP-1 in plasma， pancreas and ileum tissues are significantly increased in rats with SAP， MCP-1 aggravate the injury of pancreas and ileum tissues.