乳腺浸润性导管癌组织中COX-2和Ki-67的表达及相关性分析

目的探讨环氧化酶-2（COX-2）和Ki-67在乳腺浸润性导管癌组织中的表达情况及其临床意义。方法采用免疫组织化学sP方法检测82例乳腺浸润性导管癌组织和相应癌旁正常组织中COX．2和Ki．67蛋白表达，并分析二者表达的相关性以及其表达与乳腺浸润性导管癌患者临床病理特征的关系。结果①在82例乳腺浸润性导管癌组织中COX-2和Ki．67蛋白表达阳性率均分别明显高于其在相应癌旁正常组织中的表达阳性率[COX．2：71．95％（59／82）比8．54％（7／82），z。=68．56，P〈0．001；Ki-67：64．63％（53／82）比13．42％（11／82）。X2=45．20，P〈0．001]。②COX．2和Ki．67蛋白阳性表达与肿瘤TNM分期（cox．2：rs=O．349，P〈0．05；Ki．67：rs=0．305，P〈0．05）、淋巴结转移（cOx-2：rs=0．336，P〈0．05；Ki-67：rs=0．419，P〈0．01）、脉管侵犯（cOx-2：rs=0．235，P〈0．05 ki-67：rs=0．461，P〈0．01）及组织学分级（cox-2：rs=0．434，P〈0．01；Ki-67：rs=0．378，P〈0．05）均呈正相关；Ki．67蛋白阳性表达与肿瘤直径呈正相关（rs=0．365，P〈0．01），而COX．2蛋白阳性表达与此无关（rs=0．135，P〉0．05）；COX-2和Ki-67蛋白阳性表达皆与患者是否绝经无关（cox-2：rs=O．172，P〉0．05；Ki．67：rs=0．163，P〉0．05）。③在浸润性导管癌组织中COX．2和幻．67蛋白阳性表达呈正相关性（rs=0．475，P〈0．01）。结论在乳腺浸润性导管癌组织中COX．2和Ki．67的表达均增高，两者与乳腺浸润性导管癌临床病理特征密切相关，对两者进行联合检测可反映乳腺浸润性导管癌的生物学行为，COX-2有可能成为乳腺癌治疗的一个新靶点。

Expressions of Cyclooxygenase-2 and Ki-67 in Invasive Ductal Carcinoma of Breast Tissues and Their CorrelationAnalysis

Objective To study the expressions of cyclooxygenase-2 （COX-2） and Ki-67 in the invasive ductal carcinoma （IDC） of breast and to analyze its clinical significance. Methods Immunohistochemical SP method was performed to detect the expressions of COX-2 and Ki-67 in 82 cases of IDC of breast and corresponding tumor-adjacent normal breast tissues, and the relationship of these expressions to clinicopathologic characteristics was analyzed. Results ① The positive rates of COX-2 and Ki-67 protein expressions in the IDC of breast tissues were significantly higher than those in the corresponding tumor-adjacent normal breast tissue [ COX-2： 71.95% （59/82） versus 8. 54% （7/82）, 2＇2= 68.56, P〈0. 001 ; Ki-67： 64. 63% （53/82） versus 13.42% （11/82）, X2=45.20, P〈0. 001 ]. ②The positive rates of COX-2 and Ki-67 protein expressions were positively correlated with TNM staging （COX-2： rs=0. 349, P〈0. 05 ; Ki-67 ： rs=0. 305, P 〈 0.05）, lymph node metastasis （COX-2： rs=0. 336, P〈0.05; Ki-67： rs=0. 419, P〈0.01）, vascular invasion （COX-2： rs=0. 235, P〈0. 05; Ki-67： rs=0. 461, P〈0.01）, and histological grade （COX-2： rs=0.434, P〈 0. 01 ; Ki-67： rs=0. 378, P〈0. 05）. The positive rate of Ki-67 protein expression was positively correlated with tumordiameter （rs=0. 365, P〈0. 01）, but the positive rate of COX-2 protein expression wasn＇t correlated with it （r~=0. 135, P〉 0. 05）. The positive rates of COX-2 and Ki-67 protein expressions weren＇t correlated with menstrual status （COX-2 ： rs- 0. 172, P〉0.05; Ki-67： rs-0. 163, P〉0.05）. ③ The positive rate of COX expression was positively correlated with the positive rate of ki-67 expression （rs=0. 475, P 〈 0. 01）. Conclusions There are high-expressions of COX-2 and Ki-67 in IDC of breast. COX-2 and Ki-67 are significantly correlated with the clinicopathologic characteristics in IDC of breast. Combined detection of COX-2 and Ki-67 might calculate the biological behaviors of IDC of breast. COX-2 might be a target of molecular targeted therapy to breast cancer.