三阴性乳腺癌组织中MMP-3和VEGF表达的相关性及临床分析

目的探讨基质金属蛋白酶-3（MMP-3）和血管内皮细胞生长因子（VEGF）在三阴性乳腺癌（TNBc）中表达的相关性及临床病理体征。方法对我院2008年1月至2013年1月期间收治的112例确诊为TNBC患者的乳腺癌组织进行MMP-3和VEGF免疫组织化学检测，并与同期收治的112例luminal型乳腺癌患者进行对比。结果①TNBC和luminal型乳腺癌组织中MMP-3表达阳性率分别为90．18％（101／112）和49．11％（55／112），VEGF在其中的表达阳性率分别为84．82％（95／112）和48．21％（54／112），二者在TNBC组织中的表达阳性率明显均高于其在luminal型乳腺癌组织中的表达护〈0．05）。②TNBC组织中MMP-3和VEGF阳性表达均与患者发病年龄、绝经情况、肿瘤大小、腋窝淋巴结转移和TNM分期有关（P〈0．05）。③TNBC组织中MMP-3与VEGF的表达呈正相关（rs=0．711，P〈0．01）。④获得随访的100例TNBC患者3年内复发转移率为73．21％，5年生存率（含带瘤生存）为36．61％。结论MMP-3和VEGF与TNBC的发生、发展有着密切关系，可作为评估TNBC浸润和转移的重要生物学指标。TNBC3年内复发转移率高，5年生存率极低。干预MMP-3、VEGF等因子的表达，抑制肿瘤细胞降解基质及脉管形成，从而阻断癌细胞转移，对降低TNBC3年内复发转移率和提高5年生存率有重要作用。

Correlation Between Expressions of MMP-3 and VEGF in Three Negative Breast Cancer Tissue and Their Clinical Significance

Objective To investigate the correlation between the expressions of matrix metalloproteinase-3 （MMP-3） and vascular endothelial growth factor （VEGF） in the three negative breast cancer （TNBC） and analyze their clinicopathologic significances. Methods One hundred and twelve patients confirmed TNBC from January 2008 to January 2013 in this hospital were collected. The cases of luminal type with the similar pathological type and pathological staging were chosen as control. The expressions of MMP-3 and VEGF were detected by immunohistochemistry. Results ① The positive expression rates of MMP-3 and VEGF in the TNBC tissues were significantly higher than those in the luminal tissues MMP-3： 90. 18% （101/112） versus 49. 11% （55/112）, P〈0. 05; VEGF： 84. 82% （95/112） versus 48. 21% （54/112）, P〈 0. 05 J. ②The positive expressions of MMP-3 and VEGF in the TNBC tissues were correlated with age, menopausal status, tumor size, axillary lymph node metastasis, and TNM stage （P〈0. 05）. ③ The expression of MMP-3 was positively correlated with VEGF in the TNBC tissues （rs=0. 711, P〈 0. 01）. ④ The results of follow-up showed that the recurrence and metastasis rate within 3 years was 73.21%, the survival rate within 5 years was 36. 61% in the patients with TNBC. Conclusions There is a close relation between MMP-3 and VEGF in TNBC, the expressionsof MMP-3 and VEGF may serve as important biomarkers for evaluating invasion and metastasis in TNBC. There is a higher metastasis rate within 3 years and a lower survival rate within 5 years in TNBC. According to intervening the expressions of MMP-3 and VEGF, and inhibiting cancer cells matrix degradation and vascular formation, then cancer cells metastasis could be blocked, it may be important to reduce the 3-year recurrence rate and improve 5-year survival rate.