| miRNA-155／PU.1信号通路阻滞对大鼠骨髓来源树突状细胞成熟及移植免疫的影响 |
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| 引用本文： | 徐兴伟,丁博文,朱传荣,郑鹏,冯涛,嵇武.miRNA-155／PU.1信号通路阻滞对大鼠骨髓来源树突状细胞成熟及移植免疫的影响[J].普外基础与临床杂志,2014(2):168-172. |
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| 作者姓名： | 徐兴伟 丁博文 朱传荣 郑鹏 冯涛 嵇武 |
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| 作者单位： | [1]南京大学医学院临床学院南京军区南京总医院普通外科研究所,江苏南京210002 [2]徐州市中医院普外科,江苏徐州221000 [3]淮安市第一人民医院普外科,江苏淮安223001 |
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| 基金项目： | IL-12p70 secretion levels in the supematant were tested by ELISA method. The allogeneic T lymphocyte proliferation was tested by mixed lymphocyte reaction. The transfected cells were intravenously injected into the recipient rat on day 7 before intestinal transplantation. The survival conditions as well as pathological changes were observed in each group recipients. Results OThe surface molecules CD80, CD86, and MHC-I] in the PU.1 silent group were （27. 0＋5.6）%, （23.6 ＋4. 8） %, and （36.8 q-6.8） %, respectively; versus （74. 0 ＋ 9.4） %, （76. 5 ＋ 8.7） %, and （87.8 ＋ 1 I. 3） % in the negative control group, respectively, which were significantly lower in former and showing an in creased trend （P〈0. 05）. （~） Compared with the negative control group, IL-10 secretion level was significantly increased （P〈0.05）, IL-12p70 secretion level significantly decreased （P 〈 0.05） in the PU.1 silent group. （~） The proliferation ofT lymphocytes in the PU.1 silent group was significantly lower than that in the negative control group （P〈0. 05）. （~）When the transfected DCs were injected into the intestinal transplantation rats on day 7 before operation, the survival time was （14. 3 ＋3.3） d, （7. 8＋ 1.5） d, and （8.0＋2.5） d in the PU.1 silent group, negative control group, and control group, respectively, which in the PU. 1 silent group were significantly longer than that in the other two groups （P 〈 0.05）, and the graft pathology showed that there were mild intestinal tissue damage, lymphocyte infiltration or villus edema in the PU. 1 silent group. Coneluslon miRNA-155/PU.1 signaling pathway blockade could reduce DCs maturation and induce receptor-specific immune tolerance, which are proved both in vivo and in vitro. |
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| 摘 要： | 目的探讨miRNA-155／PU-1信号通路阻滞对骨髓来源树突状细胞（DCs）成熟及其对应用于大鼠小肠移植中的免疫功能的影响。方法利用贴壁法培养诱导DCs，针对miRNA-155／PU-1信号通路中关键转录因子PU．1基因设计并合成3对PU．1siRNA（Pu．1沉默组、阴性对照组及对照组），用脂质体法转染细胞，并筛选一对高沉默效率PU．1siRNA。流式细胞术分析PU．1沉默组、阴性对照组及对照组细胞表面CD80、CD86及主要组织相容性复合体（MHC）-II的表达；ELISA法检测上清液中IL．10及IL．12p70的水平；混合淋巴细胞反应检测对同种异体T淋巴细胞的增殖作用；在大鼠小肠移植前7d经受体尾静脉等量注射3组细胞，移植后观察各组受体存活情况及移植物病理情况。结果①DCs表面分子CD80、CD86和MHC一1I表达率在PU．1沉默组分别为（27．0±5．6）％、（23．6＋4．8）％及（36．8±6．8）％，阴性对照组分别为（74．0±9．4）％、（76．5±8．7）％及（87．8±11．3）％，PU．1沉默组均分别明显低于阴性对照组（尸〈0．05）。②PU．1沉默组IL．10水平较阴性对照组明显升高（尸〈0．05），IL-12p70则明显降低（尸〈0．05）。③PU．1沉默组DCs刺激同种异体T淋巴细胞增殖也较阴性对照组明显降低（尸〈0．05）。④将PU．1沉默组DCs术前注入受体行大鼠小肠移植后，受体平均存活时间为（14．3±3．3）d，明显长于阴性对照组的（7．8±1．5）d（尸〈0．05）和对照组的（8．0＋2．5）d（P〈0．05），且术后5d时移植物病理显示移植肠组织轻度淋巴细胞浸润和绒毛水肿。结论体内外实验表明，miRNA-155／PU．1信号通路阻滞的DCs成熟度明显受到抑制，并能稳定发挥诱导受体特异性免疫耐受的作用。
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| 关 键 词： | 树突状细胞 miRNA-155 PU 1 免疫耐受 小肠移植 |
Influences of miRNA-155/PU.1 Signaling Pathway Blockade on Rat Bone Marrow-Derived Dendritic Cell Maturationand Transplantation Immunity |
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| Institution: | XU Xing-w, DING Bo-wen, ZHU Chuan-rong, ZHENG Peng, FENG Tao, J1 Wu. 1. School of Medicine, Nanjing University, Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China; 2. Department of General Surgery, Xuzhou Chinese Medicine Hospital, Xuzhou 221000, Jiangsu Province, China; 3. Department of General Surgery, Huaian First People＇s Hospital, Huaian 223001, Jiangsu Province, China |
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| Abstract: | |
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| Keywords: | Dendritic cell miRNA-155/PU 1 Immune tolerance Intestinal transplantation |
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