Functional cloning and characterization of a multidrug efflux pump,mexHI-opmD,from a Pseudomonas aeruginosa mutant |
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| Authors: | Sekiya Hiroshi Mima Takehiko Morita Yuji Kuroda Teruo Mizushima Tohru Tsuchiya Tomofusa |
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| Affiliation: | Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan. |
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| Abstract: | We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs. |
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