| 弗氏佐剂与氢氧化铝佐剂对诱导小鼠获得性免疫应答作用的比较 |
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| 引用本文： | 潘萌,蒋浩琴,周芸,郑捷.弗氏佐剂与氢氧化铝佐剂对诱导小鼠获得性免疫应答作用的比较[J].现代免疫学,2006,26(2):98-101. |
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| 作者姓名： | 潘萌 蒋浩琴 周芸 郑捷 |
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| 作者单位： | 1. 上海交通大学医学院附属瑞金医院皮肤科,上海,200025
2. 上海交通大学医学院,上海市免疫学研究所,上海,200025 |
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| 摘 要： | 为研究、比较不同佐剂对诱导小鼠产生获得性免疫应答的不同作用,以卵清白蛋白(OVA)为抗原,分别混合完全弗氏佐剂(CFA)或Al(OH)3佐剂,对C57BL/6小鼠进行常规免疫,采用流式细胞技术对细胞内细胞因子IFN-γ和IL-4进行检测;ELISA方法对特异性抗OVA抗体滴度及抗体亚型进行了检测。结果显示在免疫后CFA组产生以IFN-γ为主的细胞因子而Al(OH)3组产生以IL-4为主的细胞因子;两组中均产生特异性抗OVA IgG抗体,但CFA组以IgG2a亚型为主,而Al(OH)3组则以IgG1亚型为主,不产生IgG2a亚型抗体。实验表明,经CFA加抗原免疫后机体产生的免疫应答以Th1型细胞免疫为主,抗体类型为IgG2a;而Al(OH)3佐剂则诱导机体产生Th2型细胞免疫应答,抗体类型为IgG1。
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| 关 键 词： | 弗氏佐剂 Al(OH)3佐剂 Th1、Th2细胞因子 抗体型别 |
| 文章编号： | 1001-2478（2006）02-0098-04 |
| 收稿时间： | 2005-09-01 |
| 修稿时间： | 2005-09-012005-11-10 |
Comparison of the role of Freund's adjuvant and aluminum hydroxide hydrate in inducing mouse adaptive immune response |
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| PAN Meng,JIANG Hao-qin,ZHOU Yun,ZHENG Jie.Comparison of the role of Freund''''s adjuvant and aluminum hydroxide hydrate in inducing mouse adaptive immune response[J].Current Immunology,2006,26(2):98-101. |
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| Authors: | PAN Meng JIANG Hao-qin ZHOU Yun ZHENG Jie |
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| Institution: | 1. Department of Dermatology, Affiliated Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, China ; 2. Shanghai Institute of Immunology, Medical College of Shanghai Jiaotong University, Shanghai 200025, China |
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| Abstract: | To compare the role of different adjuvants in inducing mouse adaptive immune response,ovalbumin(OVA) was used as an antigen,emulsified with complete Freund's adjuvant(CFA) or aluminum hydroxide hydrate Al(OH)3],to immunize C57BL/6 mouse according to the routine protocol.The detections of intracellular cytokine expression and antibody production were proceeded.It was found that the mice from CFA group showed dominant IFN-γ cytokine expression and those from Al(OH)3 group showed dominant IL-4 expression.The specific IgG antibody titer was increasing in both groups,but the dominant antibody isotype was different.IgG2a was elevated in CFA group while IgG1 was higher in Al(OH)3 group.These results indicate that CFA is a strong adjuvant for inducing type-1 immune response and Al(OH)3is for type-2 response. |
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| Keywords: | Freund's adjuvant aluminum hydroxide hydrate Th1 Th2 cytokines antibody isotypes |
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