昆明地区571例门诊患者HPV感染情况分析

目的探讨昆明地区女性子宫颈感染人乳头瘤病毒(human Papillomavirus,HPV)的基因型分布和感染率情况。为预防HPV感染和宫颈癌防治提供依据。方法以MY09/MY11为外引物,GP5+/GP6+为内引物,通过巢式PCR(n PCR)检测妇科门诊样本571例,并将扩增产物进行琼脂糖凝胶电泳,将在150bp处有条带的样本送测序,测序结果通过NCBI网站blast功能开展核酸比对,并进行基因分型。结果 535例门诊患者中阳性病例111例,阳性率20.75%,其中高危型77例,占总阳性率69.37%,低危型34例,占总阳性率30.63%。结论昆明地区女性子宫颈感染HPV最常见的亚型依次为16、58、33、35、18和31。低危型最常见的亚型依次为6、81、11、91。高危型为常见类型,占总阳性率的69.37%,16型和58型为主要高危感染亚型,6型为主要低危感染亚型。

Investigation of Genotype and Infection Rate of Human Papillomavirus in Kunming City

Objective To investigate the genotype and infection rate of human Papillomavirus (HPV) in Kunming city, and provide theoretical basis for preventing and treating uterine cervical cancer. Methods Using MY09/MY11 as outer primer, GP5⁺/GP6⁺ as inner primer, 571 gynecological outpatient samples were detected by nested PCR (nPCR), PCR products were detected through agarose gel electrophoresis followed by nucleotide sequencing. Results A total of 111 of 535 outpatient's samples were HPV positive, with the positive rate of 20.75%. Among 111 positive samples, 77 cases were high risk type (69.37% of the total positive rate), 34 cases were low risk type (30.63% of the total positive rate). The most common HPV subtype in the cervical infected population in Kunming area were subtype 16, 58, 33, 35, 18 and 31 in turn. The most common subtypes of low risk were subtype 6, 81, 11, 91 in turn. Conclusion Type 16 and type 58 were the main high risk subtypes, and type 6 was the main low risk subtype in Kunming city. High risk type was the common type among the total positive samples.