仙鹤草总黄酮纯化工艺研究

目的 优选仙鹤草总黄酮纯化工艺条件.方法 采用大孔吸附树脂法,以总黄酮含量为评价指标,分别对树脂型号、树脂径高比、上样药液浓度、上样药液速度、洗脱液浓度、洗脱剂用量进行考察.结果 优选的纯化工艺条件为选用D101型大孔树脂,上样药液浓度为0.15g/ml,上样速度为1BV/h,树脂径高比为1∶10,洗脱液为3BV 50％乙醇洗脱.固形物中总黄酮含量为31.05％.结论 该纯化工艺条件合理、稳定,可用于工业化生产.

Purification Technology of Total Flavones from Herba Agrimoniae

Objective To optimize purification technology conditions of total flavones from Herba Agrimoniae.Methods By using macroporous adsorption resin and taking the total flavones content as evaluation index,we research the resin type,diameter-height ratio of resin column,concentration of sample solution,velocity of sample solution,eluent concentration and eluent amount respectively.Results The optimum purification technology conditions were as follows：the type of macroporous adsorption resin was D101,concentration of sample solution was 0.15g/ml,velocity of sample solution was 1BV/h,diameter-height ratio of resin column was 1 ∶ 10,eluent was 3BV50％ ethanol.The total flavones content in solids was 31.05％.Conclusion The purification technology conditions is reasonable,stable and can be used in industrialized production.