聚合酶链反应在检测脑脊液病原菌中的应用

目的 应用聚合酶链反应(PCR)建立快速检查方法来检测脑脊液中是否含有病原菌.方法 对138例脑脊液标本,以病原菌16S rRNA基因为靶序列,采用1对通用引物对其进行PCR扩增检测,并同时作细菌培养.结果 138例脑脊液标本中,PCR方法检测出55例,阳性率为39.86%,培养法检出24例,阳性率为17.39%,PCR方法检出率明显高于培养法(P<0.01).结论 该方法具有快速、敏感、特异、准确等优点,是一种可靠的实验手段.

Application of Polymerase Chain Reaction in Detection of Pathogens in Cerebrospinal Fluid

OBJECTIVE To set up a quick method to detect pathogens in cerebrospinal fluid. METHODS The method of polymerase chain reaction (PCR), using a pair of universal primers targeted at the 16S rRNA gene, was adopted to amplify the DNA of bacterium. 138 clinical specimens obtained from patients were examined by PCR method and bacterial culture method. RESULTS The positive rate was 39.86% of PCR and 17.39% of culture, the outcomes had statistical significance (P<0.01). CONCLUSIONS The method of PCR is fast,sensitive, specific and accurate for detecting pathogens.