膳食n-6/n-3脂肪酸比值对小鼠淋巴细胞脂肪酸构成和功能的影响

目的观察膳食n6n3脂肪酸比值对淋巴细胞脂肪酸构成及细胞功能的影响。方法BALBc小鼠随机分为5组n6n3比值分别为1(A组)、75(B组)、15(C组)、30(D组)和正常对照组,其中实验组S∶M∶P模拟中国居民膳食脂肪酸摄入的S∶M∶P为1∶15∶1,正常对照组为AIN93G配方的1∶15∶37。基础饲料采用AIN93G配方,脂肪酸构成以食用油脂调配。饲养12周。测定小鼠T淋巴细胞功能,脾淋巴细胞脂肪酸构成、PGE2水平。结果n6n3比值接近1时,小鼠T淋巴细胞增殖活性、CD4+、CD8+T细胞比例、培养上清IL2、PGE2水平显著降低;淋巴细胞C18∶2、C20∶4、n6PUFA含量显著减少;C22∶6、C16∶1、C18∶1、总MUFA含量明显高于其他实验组。淋巴细胞C22∶6含量与淋巴细胞增殖活性显著负相关;C20∶5含量与CD4+T淋巴细胞比例、IL2水平显著负相关;C16∶1含量与CD4+、CD8+T淋巴细胞比例显著负相关。结论小鼠脾淋巴细胞的脂肪酸构成受膳食脂肪酸构成的影响;n6n3比值为1组与比值为30的膳食组相比较,小鼠T淋巴细胞增殖活性受到抑制。

Effect of dietary n-6/n-3 polyunsaturated fatty acid ratio on spleen lymphocyte's function and fatty acid composition in mice

OBJECTIVE: To study the effect of dietary n-6/n-3 PUFA ratio on spleen lymphocyte's function and fatty acid composition in mice. METHODS: Male BALb/c mice were divided into 5 groups: the dietary S : m : p was 1 : 1.5 : 1 n-6/ n-3 PUFA ratio was 1, 7.5, 15, 30 respectively, and in the control group, dietary S : M : P was 1 : 1.5 : 3.7, which was followed the AIN-93G formulation. All groups consumed purified diet based on the AIN-93G formulation. The different n-6/ n-3 PUFA ratios in diets were varied by mixing of oils. After 12 wks breeding, all mice were sacrificed, and the function and fatty acid composition of Lymphocyte, IL-2 and PGE2 concentration were measured. RESULTS: When dietary n-6/n-3 PUFA ratio approximated 1, the lymphocyte proliferation, proportions of CD4+ and CD8+ T cell, IL-2 and PGE2 concentration decreased significantly. The concentration of C18 : 2, C20 : 4, n-6 PUFA in lymphocyte decreased significantly; Meanwhile, the concentration of C22 : 6, C16 : 1, C18 : 1 and total MUFA in lymphocyte significantly higher than other groups. The concentration of C2 : 6 in lymphocytes were significantly negative correlated with the proliferation of spleen lymphocytes. The concentration of C20 : 5 in lymphocyte was significantly negative correlated with the proportions of CD4+ T cell and IL-2 level. The concentration of C16 : 1 in lymphocyte was significantly negative correlated with the proportions of CD4+ and CD8+ T cell. CONCLUSION: The fatty acid composition of diet affected that of lymphocyte in mice. Compared with the T lymphocyte function in diet with n-6/n-3 ratio approximated 30, the T lymphocyte function in mice was suppressed when dietary n-6/n-3 ratio approximated 1.