Molecular Evidence of Helicobacter Pylori Infection in Prostate Tumors |
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| Authors: | Mohammed S Al-Marhoon Allal Ouhtit Aisha O Al-Abri Krishna P Venkiteswaran Qassim Al-Busaidi Josephkunju Mathew Ibrahim Al-Haddabi Omar Shareef Shahid Aquil Khalid Rahman Intisar Al-Hashmi Ishita Gupta Shyam S Ganguly |
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| Institution: | aDepartment of Surgery, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Oman;bDepartment of Genetics, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Oman;cDepartment of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Oman;dDepartment of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Oman |
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| Abstract: | ObjectivesTo determine whether Helicobacter pylori (H. pylori) is detectable in both benign prostatic hyperplasia (BPH) and prostate cancer (PCa). Epidemiological studies have shown significant associations between infective chronic prostatitis and prostatic carcinoma. Many bacteria have been found in the prostate of patients with chronic prostatitis, BPH, and PCa.MethodsOne hundred consecutive patients with prostate diseases were enrolled in the study. Detection of H. pylori DNA in prostate tissue from patients with BPH and PCa was performed using both immunohistochemistry and PCR, and the results were confirmed by DNA sequencing. Odds ratios and the Fisher Exact test were used for the analysis of the associations between the variables.ResultsAmong the patients, 78% had BPH and 19% had PCa. While immunohistochemistry showed no positive sample for H. pylori, PCR combined with sequencing detected H. pylori DNA in prostate tissue samples from 5 patients. However, statistical analysis of the data showed that BPH and PCa are not significantly associated with the presence of H. pylori DNA in prostate tissue (odds ratio = 0.94, 95% confidence interval = 0.09–23.34, one-tailed Chi-square value = 0.660, p > 0.05). The limitation of this study was the small number of PCa patients.ConclusionsThis study provides, for the first time, molecular evidence of the presence of H. pylori DNA in prostatic tissue of patients with BPH and PCa. It paves the way for further comprehensive studies to examine the association of H. pylori infection with BPH and PCa.Key Words: Helicobacter pylori infection, Prostate cancer, Benign prostate hyperplasia, PCR |
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