Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney |
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| Authors: | Hongrui Guo Hengmin Cui Xi Peng Jing Fang Zhicai Zuo Junliang Deng Xun Wang Bangyuan Wu Kejie Chen Jie Deng |
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| Affiliation: | 1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya''an, China;2. College of Veterinary Medicine, Sichuan Agricultural University, Ya''an, China |
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| Abstract: | Here we showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase. The G2/M cell cycle arrest was accompanied by up-regulation of phosphorylated ataxia telangiectasia mutated (p-ATM), p53, p-Chk1, p-Chk2, p21 protein expression and ATM, p53, p21, Chk1, Chk2 mRNA expression, and down-regulation of p-cdc25C, cdc2, cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and the cdc25, cdc2, cyclinB, PCNA mRNA expression. |
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| Keywords: | NiCl2 G2/M cell cycle arrest mRNA expression protein expression kidney |
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