鼻息肉中免疫球蛋白E白细胞介素5和粒细胞巨噬细胞集落刺激因子的表达

目的 :探讨鼻息肉组织中免疫球蛋白E(IgE)、白细胞介素 5 (IL 5 )和粒细胞巨噬细胞集落刺激因子(GM CSF)的表达对嗜酸性粒细胞 (EOS)浸润聚集的作用及IgE与IL 5和GM CSF的关系及其意义。 方法 :应用连续两步免疫酶法 (Sandwich)检测 31例鼻息肉标本 (鼻息肉组 )和 11例下鼻甲黏膜标本 (对照组 )组织匀浆中IgE水平 ;应用酶联免疫吸附测定法检测两组组织匀浆中IL 5和GM CSF水平 ,同时观测两组组织中EOS的浸润程度。结果 :鼻息肉组IgE、IL 5和GM CSF水平均明显高于对照组 (圴 P <0 .0 1) ,且三者水平分别与鼻息肉组织中EOS浸润数呈正相关 (r分别为 0 .73、0 .5 4和 0 .4 8,均P <0 .0 1) ,且鼻息肉中IgE与IL 5和GM CSF水平呈正相关 (r分别为 0 .6 5和 0 .4 2 ,分别P <0 .0 1和P <0 .0 5 )。结论 :IgE局部水平的上调提示鼻息肉中存在局部变态反应 ,其对鼻息肉中IL 5和GM CSF的表达上调和EOS的聚集具有重要作用 ;IL 5和GM CSF在鼻息肉中高表达 ,对EOS的浸润聚集具有重要作用。

Expressions of immunoglobulin E,interleukin-5 and granulocyte-macrophage colony-stimulation factor in nasal polyps tissue

Objective:The study was designed to examine the suggested roles of Immunoglobulin E (IgE), Interleukin-5(IL-5)and Granulocyte-macrophage colony-stimulation factor (GM-CSF) in the pathogenesis of eosinophil accumulation in nasal polyps(NP) and to understand the correlations between IgE and IL-5/GM-CSF in NP.Method:Nasal polyps(n=31) and healthy nasal mucosa samples(n=11) were taken during routine endonasal surgeries. The tissue-bound IgE was measured with a sequential two-step immunoenzymatic (Sandwich) assay(Access immmnoassay system, Beckman, USA). IL-5 and GM-CSF concentrations were measured with ELISA technique in homogenized polyp tissue and in control mucosa,and the number of eosinophils was counted in the corresponding tissue with hematoxylin eosin staining.Result:The level of tissue-bound IgE, IL-5 and GM-CSF was significantly higher in NP compared with control specimens(P<0.01). There was significant correlation between tissue IgE/IL-5/GM-CSF and the number of eosinophils in the NP group(r=0.73,0.54,0.48, P<0.01). The correlation between concenstration of IgE and IL-5/GM-CSF was also significant in nasal polyp group(r=0.65,0.42, P<0.01 and P<0.05).Conclusion:This study demonstrated that the tissue-bound IgE, IL-5 and GM-CSF played an important role in the recruitment of eosinophils in NP. A local upregulation of the IgE synthesis in comparision with control group suggests that the immunologic pathway or local allergy involved in the recruitment of eosinophils in NP.A local upregulation of the IgE may play an important role in the upregulation of IL-5 and GM-CSF in NP.