| 刀豆蛋白A诱导鼠肝损伤的荧光组织化学和超微病理研究 |
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| 引用本文： | 王天才,李小刚,武忠弼,李绍白,唐望先,刘绍春. 刀豆蛋白A诱导鼠肝损伤的荧光组织化学和超微病理研究[J]. 华中科技大学学报(医学版), 1997, 0(3) |
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| 作者姓名： | 王天才 李小刚 武忠弼 李绍白 唐望先 刘绍春 |
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| 作者单位： | 同济医科大学附属同济医院肝病研究所!武汉，430030，同济医科大学附属同济医院肝病研究所!武汉，430030，同济医科大学实验医学研究中心超微病理研究室!武汉，430030，同济医科大学附属同济医院肝病研究所!武汉，430030，同济医科大学附属同济医院肝病研究所!武汉，430030，同济医科大学 |
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| 摘 要： | 观察了刀豆蛋白A（ConA）诱导小鼠急性肝损伤模型的荧光组织化学和超微病理改变。小鼠注射ConA后，经超微病理观察发现，肝损伤突出表现为肝细胞凋落；用生物素化的ConA注射于小鼠，切肝制成冰冻切片，再加入异硫氰荧光素（FITC）标记的亲合素可见大部分荧光分布于肝脏。结果表明，细胞凋落可能是ConA在体内造成肝细胞死亡的机制；ConA具有亲肝特性。
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| 关 键 词： | 刀豆蛋白A 肝损伤 模型.生物学 |
Fluorescent Histochemical and Ultrastructural Pathological Study on Concanavalin A-induced Liver Injury in Mice |
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| Wang Tiancai, Li Xiaogang, Wu Zhongbi et al. Fluorescent Histochemical and Ultrastructural Pathological Study on Concanavalin A-induced Liver Injury in Mice[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1997, 0(3) |
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| Authors: | Wang Tiancai Li Xiaogang Wu Zhongbi et al |
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| Abstract: | The purpose of this study was to examine changes in fluorescent histochemistry and ultrastructural pathology on concanavalin A (Con A)-induced liver injury in mice. Hepatic injury was characterized by apoptosis of liver cells through observation of electron microscope after Con A challenge. Livers were removed and divided into frozen sections after intravenous administration of Con A biotinylated to mice.When fluorescein isothiocyanate (FITC)-labeled Avidin was administered in the sections, the major fluorescence was found within the liver. This suggests that apoptosis is a possible mechanism of hepatocyte cell death induced by Con A in vivo and the distribution of Con A is specific for the liver. |
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| Keywords: | concanavalin A liver injury models biological |
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