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| 循环牵张应力对人退变纤维环细胞增殖的影响 | |
| 引用本文： | 李爽,马信龙,孙晓雷,李秀兰,马剑雄,张扬,郭悦,宁尚龙. 循环牵张应力对人退变纤维环细胞增殖的影响[J]. 国际生物医学工程杂志, 2011, 34(6): 344-347,361,F0003. DOI: 10.3760/cma.j.issn.1673-4181.2011.06.006 |
| 作者姓名： | 李爽 马信龙 孙晓雷 李秀兰 马剑雄 张扬 郭悦 宁尚龙 |
| 作者单位： | 1. 300052,天津医科大学总医院骨科 2. 300052 天津医科大学总医院骨科;300211 天津市天津医院 3. 300211,天津市天津医院 |
| 基金项目： | 国家青年科学基金资助项目，天津市卫生局重点基金资助项目 |
| 摘 要： | 目的 探讨不同水平循环牵张应力对人退变椎间盘细胞增殖的影响.方法 取1例29岁手术切除的腰椎间盘突出患者的椎间盘,通过病理学方法评价其退变程度,酶消化法分离椎间盘纤维环细胞.选取P3代细胞,利用ElectroForce 3200力学试验仪搭载的BioDynamic生物反应舱系统,以3h为固定时间点对其进行不同水平的频率... |
| 关 键 词： | 椎间盘退变 循环牵张应力 纤维环细胞 增殖指数 |
Response of cyclic tensile strain on the human degenerative anulus fibrosus cells |
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| LI Shuang,MA Xin-long,SUN Xiao-lei,LI Xiu-lan,MA Jian-xiong,ZHANG Yang,GUO Yue,NING Shang-long. Response of cyclic tensile strain on the human degenerative anulus fibrosus cells[J]. International Journal of Biomedical Engineering, 2011, 34(6): 344-347,361,F0003. DOI: 10.3760/cma.j.issn.1673-4181.2011.06.006 | |
| Authors: | LI Shuang MA Xin-long SUN Xiao-lei LI Xiu-lan MA Jian-xiong ZHANG Yang GUO Yue NING Shang-long |
| Affiliation: | . (Department of Orthopaedics, General Hospital of Tianjin Medical University, Tianjin 300052, China) |
| Abstract: | Objective To investigate the effects of different cyclic tensile strain on the proliferation of human anulus fibrosus cells from degenerated discs.Methods Anulus fibrosus(AF) cells were isolated from a degenerated human IVD,expanded in monolayer,and cyclically strained for 3 hours,applying 0,5％,10％,15％ and 20％ strains at a frequency of 0.25 Hz with the use of the DioDynamic test instrument.The flow cytometry method was used to examine the Af cells proliferation at 24 hours following application of the cyclic tensile strains.Results The proliferative index (PI) increased with the magnitude value of cyclic tensile strain except 20％ group.The most significant increase of proliferation index were found in 15％ group.Conclusion There might be some corelationships between magnitude of cyclic tensile strain and the proliferation of the degenerative AF cells. |
| Keywords: | Intervertebral disc degeneration Cyclic tensile strain Anulus fibrosus cells Proliferative |
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