黄芪多糖增强小鼠免疫功能的实验研究

目的 研究黄芪多糖对小鼠免疫功能的影响.方法 采用ConA诱导小鼠淋巴细胞转化试验及迟发性变态反应试验测试黄芪多糖(Astragalus Polysaccharide APS)对小鼠细胞免疫功能的影响;采用抗体生成细胞检测及半数溶血值实验测试黄芪多糖对小鼠体液免疫的影响;采用小鼠腹腔巨噬细胞吞噬鸡红细胞试验测试黄芪多糖对小鼠非特异性免疫的影响.结果 黄芪多糖能明显提高小鼠的足跖肿胀度、脾脏生成抗体细胞数、半数溶血值及巨噬细胞吞噬功能,黄芪多糖对小鼠淋巴细胞增殖能力无明显影响.结论 黄芪多糖对正常小鼠的细胞免疫、体液免疫及巨噬细胞功能均有明显的增强作用.

Effect of Astragalus Polysaccharide on Immunological Function in Mice

Objective To investigate the effect of astragalus polysaccharide (APS) on immunological function in mice.Methods By lymphocyte transformation test and delayed type hypersensitivity (DTH),the effect of APS on celluar immunity was evaluated.By determining plaque forming cells (PFC) and HC50,the effect of APS on humoral immunity was studied.Abdomen macrophages of mice devour chicken red cell was used to asscess phagositosis of peritoneal macrophages.Results Compared with the normal control group,APS could promote DTH,PFC,HC50 and phagositosis of peritoneal macrophages,but had no effect on lymphocyte proliferation.Conclusion APS can stimulate the immunological function in mice in different ways.