平阳霉素对呼吸道黏膜的影响及其预防探讨

目的　观察平阳霉素对健康Wistar大鼠鼻和气管黏膜组织形态、超微结构的影响并探讨维生素E(VitE)、复方丹参注射液对它的干预作用。方法　健康Wistar大鼠 3 0只采用随机数字表法分为 4组 ,甲组 :对照组 (腹腔注射 0 9%氯化钠溶液 ) ;乙组 :平阳霉素腹腔注射组 ,每次 1 0mg/kg体重每周 2次 ,共 4周 ;丙组 :平阳霉素腹腔注射 +VitE灌胃组 ;丁组 :平阳霉素腹腔注射 +复方丹参注射液腹腔注射组。于 1、2、4、6周取鼻和气管黏膜行光镜和电镜观察。结果　乙组在第 1周见细胞水肿、变性 ,表现为内质网扩张 ,线粒体肿胀 ,嵴排列紊乱 ,线粒体空泡化 ,部分细胞线粒体减少 ,核周间隙轻度扩大 ,染色质轻度边集等。第 2周见上皮细胞坏死、脱落 ,细胞膜破裂 ,线粒体、内质网等细胞器外溢。第 4周细胞受损伤程度更加重 ,停药 2周后仍呈较重损伤。丙、丁 2组均可见细胞受损伤程度明显减轻 ,且出现时间推迟 ,停用平阳霉素并续用VitE、复方丹参注射液 2周可见细胞无明显改变 ,与甲组相似。结论　平阳霉素可损伤健康Wistar大鼠鼻和气管黏膜 ,并随剂量增加而损伤程度加重 ;VitE、复方丹参注射液均可减轻平阳霉素对Wistar大鼠鼻和气管黏膜的损害 ,对平阳霉素的呼吸道黏膜损伤具有保护作用

Effects of pingyangmycin on mucous membrane of respiratory tract and its prevention

Objective To observe the effects of pingyangmycin(PYM) on the histomorphology and ultrastructure of airway mucosa from the healthy Wistar rats and explore the intervenient role of Vitamin E and Composite Salvia Miltiorrhiza (CSM)on these effects Methods Thirty Wistar rats were divided randomly into four groups: Group A as control group (injection of N S into peritoneal cavity); Group B received intracavitary injection of PYM; Group C received both intracavitary injection of PYM and tube feeding of Vitamin E; Group D received intracavitary injection of PYM and CSM The nasal and tracheal mucosa were taken for light microscopy(LM),transmission electron microscopy(TEM) and scanning electron microscopy(SEM) at the 1st?2nd?4th and 6th week of the experiment Results Edema and degeneration of epithelial cells manifested as expansion of endoplasmic reticula, swelling of mitochondria, disarrangement of mitochondrial crista , vesicula formation of mitochondria, reduction of mitochondria in part cells, mild expansion of perinuclear space and slight peripheral accumulation of nuclear chromatin could be seen in Group B at the end of the 1st week without necrosis or detachment of the cells At the 2nd week, there were necrosis and exfoliation of epithelial cells, rupture of cell membrane, phenomena of outflow of mitochondria and endoplasmic reticula The degree of cell damage became more seriously at the 4th week and was not recovered to normal condition even 2 weeks after stopping administration of PYM The cell damages in Group C and D were significantly lighter than that in Group B and its occurrence were significantly postponed The injured cells recovered definitely after stopping PYM administration and continuous application of Vitamin and CSM for another 2 weeks Conclusions (1) The nasal and tracheal mucosa of Wistar rats can be damaged by injection of PYM and the degree of damage which become more serious with the increase of dose (2) Vitamin E and CSM can alleviate the damages of the respiratory mucosa from Wistar rats caused by PYM Both drugs could be used to prevent respiratory tract mucosa from the damages induced by PYM in clinical