隐丹参酮在小肠吸收机制的实验研究

目的研究隐丹参酮在大鼠小肠的吸收机制。方法用大鼠在体一过式肠灌流方法，通过肠系膜静脉插管采集血样，同时收集灌流流出液，以高效液相色谱法测定样品中隐丹参酮含量，计算其通透率。结果隐丹参酮在大鼠小肠的肠管通透率(Plumen)和血管通透率(Pbtood)随浓度的增加而下降；加入P-糖蛋白抑制剂维拉帕米后其通透率增高。结论隐丹参酮在大鼠小肠的转运机制可能为主动转运，隐丹参酮可能为P-糖蛋白底物。

Experimental study on the absorption mechanism of cryptotanshinone single -pass intestinal perfusion model

Objective To study the absorption mechanism of cryptotanshinone in the rat intestine. Methods The in situ rat single-pass intestinal perfusion model with mesenteric vein cannulated was used. Samples were obtained continuously from the outlet perfusate and the mesenteric vein. The cryptotanshinone was determined by high-performance liquid chromatography, and then the permeability coefficients were calculated. Results The permeability values decreased at the higher cryptotanshinone concentration, which suggested a saturable transport process. And the permeability significantly increased when cryptotanshinone was perfused with P-glycoprotein inhibitor verapamil. Conclusion The absorption of cryptotanshinone across rat ileum is similar to an active transport mechanism, and P-glycoprotein may play a role in the absorption.