| HPLC法测定抗感解毒胶囊中葛根素的含量 |
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| 引用本文： | 赵昌军,陈雯.HPLC法测定抗感解毒胶囊中葛根素的含量[J].齐鲁药事,2010,29(6):334-336. |
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| 作者姓名： | 赵昌军 陈雯 |
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| 作者单位： | 淄博市药品检验所,山东,淄博255000 |
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| 摘 要： | 目的建立抗感解毒胶囊中葛根素的高效液相色谱含量测定方法。方法采用HPLC法,C18色谱柱(250mm×4.6mm,5μm),以甲醇-水(25∶75)为流动相,流速:1.0mL·min-1,检测波长:250nm。结果葛根素浓度在4.008～200.4μg·mL-1的范围内与峰面积呈良好的线性关系,Y=0.0126X+0.0141,r=1.000(n=6),平均回收率为99.95%,RSD=0.19%。结论该方法简便、准确,可作为抗感解毒胶囊的质量控制方法。
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| 关 键 词： | 抗感解毒胶囊 葛根素 高效液相色谱法 含量测定 |
Determination of puerarin in Kangganjiedu Capsule by HPLC |
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| ZHAO Chang-jun,CHEN Wen.Determination of puerarin in Kangganjiedu Capsule by HPLC[J].qilu pharmaceutical affairs,2010,29(6):334-336. |
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| Authors: | ZHAO Chang-jun CHEN Wen |
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| Institution: | (Zibo Institute for Drug Control,Zibo 255000) |
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| Abstract: | OBJECTIVE To establish a method for determination of puerarin in Kangganjiedu Capsule by HPLC. METHODS The chromatographic column of ODS C18 （250mm ×4. 6mm,5μm） was used,the mobile phase was consisted of methanol-water （25 ： 75）,the flow rate was 1.0mL ·min^-1 ,the detective wavelength was 250nm. RESULTS The linear range of the method was 4. 008-200.4μg （r=1. 000, n = 6）, the average recovery was 99. 95%, RSD was 0. 19% CONCLUSION The method was simple,accurate,which can be used in quality control of Kangganjiedu Capsule. |
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| Keywords: | Kangganjiedu Capsule puerarin HPLC determination |
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