HPLC与UV测定阿司匹林肠溶片中阿司匹林和水杨酸的含量

目的：准确测定阿司匹林肠溶片的含量。方法：HPLC测定：采用Kromasil C18色谱柱;以甲醇∶醋酸∶水（40∶1∶60）为流动相;流速：1.0 mL/min,检测波长：276 nm。紫外法测定：采用双波长紫外分光光度法,测定波长分别为276 nm、322 nm。结果：HPLC测定：阿司匹林线性范围：0.67～10.05μg/mL（r=0.9997）,平均回收率为100.7%,RSD 0.5%。水杨酸线性范围：2.74～29.68μg/mL（r=0.9994）,平均回收率为102.1%,RSD 1.5%。紫外法测定：阿司匹林的平均回收率为100.1%,RSD0.25%。结论：此两种方法能有效地消除阿司匹林水解产物水杨酸对测定的干扰,简便、快速、准确。

Determination of Aspirin Enteric-coated Tablets by HPLC and UV Spectrophotometry

Objective：Accurate Determination of the content of aspirin in aspirin enteric-coated tablets.Methods：HPLC： Diamonsil C18 column;methanol-acetic acid-water（40∶1∶60） as mobile phase;flow rate：1.0 mL/min,detection wavelength of 276 nm;Dual-wavelength Spectrophotometric Determination of wavelength of 276 nm and 322 nm.Results：HPLC：Aspirin linear range：0.67～10.05 μg/mL（r=0.9997）,the average recovery was 100.7%,RSD 0.5%.Salicylic acid linear range：2.74～29.68 μg/mL（r=0.9994）,the average recovery was 102.1%,RSD was 1.5%.UV：The average recovery of aspirin 100.1%,RSD was 0.25%.Conclusion：The two kinds of methods can effectively eliminate the aspirin hydrolysis product of the interference on the determination of salicylic acid,is simple,rapid,accurate.