外周血CEAmRNA、CEA及VEGF检测在诊断肿瘤微转移中的意义

目的探讨检测外周血癌胚抗原信使核糖核酸(CEAmRNA)表达、血清血管内皮细胞生长因子(VEGF)及血清癌胚抗原(CEA)水平在诊断恶性肿瘤微转移中的临床意义。方法36例经病理确诊的恶性肿瘤患者(其中13例临床确诊远处转移,23例无确切临床转移征象),17例健康体检对照者,采用巢式逆转录聚合酶链反应(nestRT-PCR)检测外周血CEAmRNA表达,并联合检测血清VEGF水平(酶联免疫吸附法,ELISA)及血清CEA的水平(化学发光法)。结果13例远处转移患者中CEAmRNA表达阳性11例(84.6%),23例未转移患者中CEAmRNA表达阳性6例(26.1%),17例健康对照组CEAmRNA表达均阴性,肿瘤转移病人外周血CEAmRNA表达明显高于无转移病人;血清CEA在远处转移患者与未转移患者中的差异无统计学意义;远处转移患者的VEGF水平高于未转移患者及健康对照者。联合检测外周血CEAmRNA表达及血清VEGF水平在肿瘤转移诊断中有更高的敏感性。结论联合检测外周血CEAmRNA表达及血清VEGF水平可能有助于恶性肿瘤微转移的早期诊断。

Clinical significance of detecting CEAmRNA, CEA and VEGF in peripheral blood on diagnosing the micrometastasis of malignant tumors

Objective:To study the clinical significance that detect the express of CEAmRNA of peripheral blood,VEGF of blood serum,and CEA of blood serum on diagnosing the micrometastasis of malignant tumors.Methods:There were 36 cases of malignant tumors diagnosed by the pathology (13 cases with distant metastasis,23 cases without metastasis),17 health volunteers,applying the nest RT-PCR to detect the express of CEAmRNA of peripheral blood,and combined detecting the VEGF of blood serum (ELISA),and CEA of blood serum (chemoluminescence technique).Results:For the 13 cases with distant metastasis,the express of CEAmRNA was masculine in 11 cases (84.6%),for the 23 cases without metabasis,the express of CEAmRNA was masculine in 6 cases (26.1%),for the 17 health volunteers,the express of CEAmRNA was all negative. the express of CEAmRNA of peripheral blood in the tumor patients with metastasis was obviously higher that the tumor patients without metastasis; the differences of CEA between the cases with and without distant metabasis had not statistical significance; the VEGF of the cases with distant metastasis was higher than the cases without metastasis and the health volunteers.Conclusion:Combined detecting the express of CEAmRNA of peripheral blood and VEGF of blood serum is probably beneficial for the early diagnosis of the micrometastasis of malignant tumors.