内源性肾上腺糖皮质激素对血管炎症反应的抑制作用

目的：探讨内源性肾上腺糖皮质激素时脂多糖（lipopolysaccharide,LPS）诱导大鼠血管炎症因子IL-6、IL-β、IL-10表达、促炎转录因子NF-kB活性以及HDAC活性的影响及其机制.方法：分别给予大鼠RU486（肾上腺糖皮质激素拮抗刺）、TSA（HDAC抑制剂）和摘除双侧肾上腺处理后,腹腔注射LPS,取其主动脉提取组织总蛋白,ELISA法检测IL-1β、IL-6、IL-10含量及NF-κB、HDAC活性.结果：与正常对照组相比,LPS诱导大鼠主动脉IL-1β、IL-6、IL-10含量以及NF-κB活性升高（P＜0.05）;分别给予RU486、TSA以及摘除双侧肾上腺处理再腹腔注射LPS,与单纯给予LPS相比,IL-1β、IL-6、IL-10含量以及NF-κB活性均显著升高（P＜0.05）.给予RU486或摘除双侧肾上腺处理对大鼠主动脉组织中HDAC的活性未见明显影响;而给予TSA处理,大鼠主动脉中HDAC的活性受到抑制（P＜0.05）.结论：内源性肾上腺糖皮质激素同样具有抗炎作用,尤其是对血管炎症反应的抑制作用,而核组蛋白去乙酰化酶HDAC可能对致炎因子的表达具有负调控作用,可以从转录水平抑制血管炎症因子的合成.

The inhibitory effect of endogenous glucocorticoid on vascular inflammation

Objective：To study the inhibitory effects of endogenous glucocorticoid on vascular inflammation. Methods：IL-1β ,IL-6, IL-10, NF-κB and HDAC of thoracic aorta in rats ,which were pretreated with RU486 ,TSA or adrenaleetomy and then received LPS, were tested by ELISA. Results： Respectively pretreated with RU486, TSA and adrenaleetomy markedly enhanced the effect of LPS on the expression of IL-1β, IL-6, IL-10 and NF-κB of rat thoracic aortas （ P 〈 O. 05 ）. Preteated with TSA can inhibit the activity of HDAC （ P 〈 0.05 ）, but RU486 or adrenaleetomy had no obvious effect. Conclusion： Endogenous gleeocorticoid had the anti-inflammatory effect on vascular inflammation induced by LPS in rats, and HDAC may be have a negative regulation effect on the expression of cytokine.