IDA对白血病骨髓基质培养体系中HL-60细胞杀伤效应的观察

目的 :研究细胞毒药物对体外残留白血病模型中HL 6 0细胞的杀伤效应。方法 :采用Dexter型骨髓培养体系形成白血病骨髓基质细胞贴壁层 ,接种HL 6 0细胞共培养 ,用去甲氧基柔红霉素 (IDA)处理后 ,动态观察HL 6 0细胞的活性变化。结果 :随着IDA剂量的增加及培养时间的延长 ,HL 6 0细胞活力逐渐减弱 ,去甲氧基柔红霉素 (IDA)与骨髓基质细胞层或单纯的培养基体外孵育对HL 6 0细胞杀伤能力减弱。结论 :急性白血病骨髓基质有助于HL 6 0细胞逃避化疗药物杀伤 ,对残留白血病的形成具有重要作用。

Influence on the Vigor of HL-60 Cells Killed by IDA in Acute Leukemia Stroma

Objective To study the mechanism of acute leukemic bone marrow stroma in leukemia development.Methods The growth properties of leukemic bone marrow stroma were investigated with the method of bone marrow stromal cell culture system or co culture system of bone marrow stromal cells,HL 60 cells and idarubicin,flow cytometry and enzyme linked immunoadsodent assay respectively.The roles of acute leukemic bone marrow stromal cell in residual leukemia development were also investigated.Results The action of acute leukemic bone marrow stromal cells on proliferation of HL 60 cells significantly increased,while that on induction differentiation significantly decreased compared with that of the controls.The capacity for binding of HL 60 cells to acute leukemic stromal cell adherence layer significantly increased compared with that of the controls.The effect of IDA was dosage and time dependency.Conclusions Bone marrow stromal cell or stromal cell conditioned culture medium had no roles in inactivation IDA and effect on HL 60 cells of IDA was weakened in vitro incubation.The residual leukemia development in some degree was attributed to the role of leukemic bone marrow stroma.