一种简单实用纯化腹水McAb方法--辛酸/硫酸铵法

采用辛酸－硫酸铵法（ＣＡ－ＡＳ）、硫酸铵沉淀法（ＣＡ）和亲合层析法，分别提取了小鼠多株腹水单抗。结果显示：ＣＡ－ＡＳ法提取的ＩｇＧ 纯度为８７％ ～９１％ ，高于ＣＡ 法（６５％ ～７０％ ），低于亲合层析法（１００％ ）；ＣＡ－ＡＳ法提取的ＩｇＧ得率为４．８～５．５ｇ／Ｌ腹水，低于ＣＡ 法（７．０～７．９ｇ／Ｌ腹水），远高于亲合层析法（０．６ｇ／Ｌ腹水）；ＣＡ－ＡＳ法提取ＩｇＧ回收率为３９．０％ ～４４．４％ ，低于ＣＡ法（５６．０％ ～６５．０％ ），远高于亲合层析法（７．４％ ）。４Ｅ３、Ｈ６ 腹水抗体经ＣＡ－ＡＳ法提取前后酶联效价不变，表明ＣＡ－ＡＳ法对ＭｃＡｂ 活性无影响。作者认为，ＣＡ－ＡＳ法是一种操作简便，耗时短，效果佳，成本低的提取腹水ＭｃＡｂ 的新方法。

One Simple and Efficient Method for Purification of IgG McAb from Mice Ascites:Caprylic Acid/Ammonium Sulfate Precipitation

Several ascitic IgG McAbs were purificated by caprylic acid/ ammonium sulfate precipitation (CA AS), ammonium sulfate precipitation (AS) and SPA affinity chromatography. Purity of IgG purificated by CA AS ranged from 87% to 91%, higher than that obtained by CA (65%~70%), lower than that obtained by SPA affinity chromatography (100%). The yield of IgG purificatied by CA AS ranged from 4.8 g/L to 5.5 g/L, lower than that obatined by CA (7.0~7.9g/L), more higher than that obtained by SPA affinity chromatography (0.6g/L). The recovery of IgG purificatied by CA AS ranged from 39.0% to 44.4%, lower than that obtained by CA (56.0%~65.0%), more higher than that obtained by SPA affinity chromatography (7.4%). The ELISA titer of 4E 3 or H 6 McAb was not impaired by purification of CA AS. The above results suggested that CA AS is one simple, efficient, rapid and low cost method for purification of IgG McAb from ascitic fluid.