痹速清合剂对佐剂性关节炎大鼠血清细胞因子水平的影响

目的 :观察痹速清合剂对佐剂性关节炎的治疗作用及对细胞因子系列 (IL 1β、TNF α、IL 4、IL 10 )的影响。方法 :将 4 0只雄性Wistar大鼠随机分为正常对照组、模型对照组、消炎痛治疗组及痹速清合剂治疗组 ,采用佐剂性关节炎 (AA)大鼠模型 ,观察各组对AA大鼠足肿胀、血清细胞因子含量及体重的影响。结果 :痹速清合剂与消炎痛均能显著抑制AA大鼠原发性及继发性关节肿胀 ,显著降低AA大鼠血清IL 1β、TNF α水平 ,升高IL 4、IL 10水平。在降低IL 1β、TNF α水平 ,增加AA大鼠体重方面作用优于消炎痛 (P <0 .0 5 )。结论 :痹速清合剂对佐剂性关节炎大鼠有明显的治疗作用 ,其作用机制可能是通过下调致炎因子 (IL 1β、TNF α)水平、上调抗炎因子 (IL 4、IL 10 )水平、抑制致炎效应、增强抗炎效应而实现的。

INFLUENCE OF BISUQING MIXTURE UPON SERUM CYTOKINE LEVELS IN RATS WITH ADJUVANT ARTHRITIS

Objective: To observe the curative effects of Bisuqing mixture(BM) on adjuvant arthritis(AA) rat and explore the influence of BM upon serum IL-1β, TNF-α, IL-4 and IL-10.Methods: The experiment applied AA rat as pathological model. Forty male Wistar rats were randomly divided into four groups: normal control, AA control, AA with indometacin and AA with BM. Results: BM and indometacin can significantly inhibit the original and secondary arthritis of AA rat, lower the IL-1β and TNF-α levels,and increase the IL-4 and IL-10 levels in serum. BM had better effect in reducing the serum IL-1β and TNF-α levels, increasing the weight of AA rats than those of indometacin. Conclusions: BM is effective for AA rat. The mechanisms may be related to reducing the IL-1β and TNF-α levels, increasing the IL-4 and IL-10 level, suppressing pathogenic effect and enhancing anti-inflammatory activity.