刺五加注射液对化疗药物心脏损伤保护及辅助治疗的实验研究

目的 研究刺五加注射液对化疗药物阿霉素诱导心肌损伤的保护及助眠、抗抑郁作用，为其临床的辅助治疗应用提供实验依据。方法 1）大鼠ip 2.5 mg/kg的盐酸阿霉素，每周1次，连续6周，诱导大鼠心脏毒性模型；于阿霉素注射后立即尾静脉输注药液，时长1 h，共6次，对照组、模型组给予生理盐水，受试药组给予刺五加注射液（50、100、200 mg/kg，以总黄酮计），阳性对照组给予参芪扶正注射液（25 mL/kg）；测定大鼠血清心肌酶、炎性因子，以及心肌组织病理改变。2）以地西泮（0.5 mg/kg）为阳性对照，测定刺五加注射液（30、60、120 mg/kg）对戊巴比妥钠阈剂量（45 mg/kg）及阈下剂量（30 mg/kg）致小鼠睡眠的影响。3）采用小鼠悬尾实验，以氟西汀（20 mg/kg）为阳性对照，测定刺五加注射液（30、60、120 mg/kg）对不动时间的影响，观察其抗抑郁作用。结果 与模型组比较，刺五加注射液50、100、200 mg/kg不同程度减轻心肌病理改变，减少心肌酶乳酸脱氢酶（LDH）、肌酸激酶同工酶MB（CK-MB）漏出，除刺五加注射液50 mg/kg组CK-MB外，均差异显著（P<0.05）；可使IL-1β、IL-6、TNF-α水平不同程度降低，其中100、200 mg/kg组均差异显著（P<0.05、0.01）。与对照组比较，刺五加注射液30、60、120 mg/kg剂量相关性地显著缩短小鼠睡眠潜伏期（P<0.05、0.01、0.001），120 mg/kg组显著延长睡眠时间（P<0.001），60、120 mg/kg组显著增加入睡率（P<0.05、0.01）。与对照组比较，刺五加注射液各剂量组明显缩短小鼠悬尾不动时间（P<0.05、0.01）。结论 刺五加注射液对化疗药物阿霉素导致的心肌损伤治疗效果明显，而且能助眠、抗抑郁，这些作用对于恢复机体体力、促进健康有重要作用。

Effect of Acanthopanax Injection on myocardial protection induced by doxorubicin in rat and sleeping, antidepressant effect in mice

Objective To investigate the protective effect of Acanthopanax Injection on cardiotoxity induced by doxorubicin in rats, and the hypnotic effect together with antidepressant effect of Acanthopanax Injection was researched in mice. Methods 1) The cardiotoxicity model of rats was induced by ip injection of doxorubicin hydrochloride with 2.5 mg/kg once a week for 6 Weeks. The total cumulative dose was 15 mg/kg. The serum myocardial enzymetissue were determined after Acanthopanax Injection treatment. 2) The sleep-promoting effects of Acanthopanax Injection on the threshold dose (45 mg/kg) and subthreshold dose (30 mg/kg) of sodium pentobarbital were studded in mice. 3) The time of immobility is recorded in the tail suspension test (TST) in mice. The normal group and model group were given NS, and the Acanthopanax Injection groups were given 50, 100, 200 mg/kg in the rats, and 30, 60, 120 mg/kg in the mice respectively; the positive control was given Shenqi Fuzheng Injection 25 mL/kg in rats, diazepam 0.5 mg/kg, and fluoxetine 20 mg/kg in mice,respectively. Results After treatment with 50, 100, and 200 mg/kg of Acanthopanax Injection in rats,myocardial pathological changes were reduced to different degrees, with less leakage of myocardial enzyme LDH and CK-MB. As the mice treated by Acanthopanax Injection at the dose of 30, 60, 120 mg/kg, the sleep incubation period shortened,the sleep time prolonged, and the sleeping rate increased. The time of immobility was significantly shortened in TST with Acanthopanax Injection administration. Conclusions Acanthopanax Injection has a protective effect of on cardiotoxity induced by doxorubicin in rats, Acanthopanax Injection also shows hypnotic and antidepressant effects in mice, which are important for restoring the body''s physical strength and promoting health.