大株红景天对糖尿病视网膜病变大鼠眼血流影响的实验研究

目的：探讨大株红景天对糖尿病视网膜病变(DR)大鼠眼血流的影响。

方法：将90只SD大鼠随机分为对照组、模型组和干预组各30只。模型组和干预组大鼠高糖高脂饲料喂养+链脲佐菌素(40mg/kg)腹腔注射，以构建DR模型大鼠，同时对照组大鼠基础饲料喂养+等量生理盐水腹腔注射。造模成功后，干预组大鼠注射大株红景天注射液(10mL/次，1次/d)，对照组和模型组大鼠注射等量生理盐水。连续干预治疗4wk。采用Laser Doppler Flowmetry激光血流仪检测大鼠眼部血流，采用TUNEL法检测大鼠视网膜细胞凋亡，采用 RT-PCR法和Western blot法检测大鼠视网膜组织血管内皮生长因子(VEGF)、胶质纤维酸性蛋白(GFAP)和谷氨酸/天冬氨酸转运体(GLAST)表达。

结果：模型组和干预组大鼠全血粘度、全血粘度高切、全血粘度低切和血沉高于对照组(P<0.01)，而干预组大鼠各指标较模型组降低(P<0.01)。模型组和干预组大鼠PSV和EDC低于对照组(P<0.01)，RI高于对照组(P<0.01)； 而干预组大鼠PSV和EDC较模型组增加(P<0.01)，RI较模型组降低(P<0.01)。模型组和干预组大鼠视网膜细胞凋亡率大于对照组(P<0.01)，而干预组凋亡率较模型组降低(P<0.01)。模型组和干预组大鼠视网膜组织VEGF和GFAP 表达高于对照组(P<0.01)，GLAST表达低于对照组(P<0.01)； 而干预组大鼠VEGF和GFAP表达较模型组降低(P<0.01)，GLAST表达较模型组增加(P<0.01)。

结论：大株红景天注射液能显著改善糖尿病视网膜病变模型大鼠眼部血液流变学和血流动力学，减少模型大鼠视网膜细胞凋亡，下调视网膜组织VEGF和GFAP表达，上调GLAST表达。

Effects of Rhodiola sachalinensis on ocular blood flow in diabetic retinopathy rats

AIM: To investigate the effects of Rhodiola sachalinensis on ocular blood flow in diabetic retinopathy rats.

METHODS: A total of 90 SD rats were randomly divided into control group(n=30), model group(n=30)and intervention group(n=30). Rats in the model group and intervention group were fed with high glucose and high fat diet and injected with streptozotocin(40mg/kg)in order to construct DR model rats, while rats in the control group were fed with basic diet and injected with the same amount of normal saline. After 4wk, the rats in the intervention group were injected with Rhodiola sachalinensis injection(10mL, one per day), while rats in the control group and the model group were injected with normal saline. The course of intervention treatment was 4wk. The ocular blood flow in rats was detected by laser doppler flowmetry, the apoptosis of retinal cells in rats was detected by TUNEL method, the expression of vascular endothelial growth factor(VEGF), glial fibrillary acidic protein(GFAP)and glutamate/aspartate transporter(GLAST)in retina of rats was detected by RT-PCR method and Western blot method.

RESULTS: The whole blood viscosity, whole blood high shear viscosity, low shear blood viscosity and erythrocyte sedimentation of rats in the model group and intervention group were higher than those in the control group(P<0.01), while the indexes of intervention group were lower than that of model group(P<0.01). The PSV and EDC of rats in the model group and intervention group were lower than those in the control group(P<0.01), and the RI was higher than that in control group(P<0.01). The PSV and EDC in the intervention group were higher than those in the model group(P<0.01), and RI was lower than that in the model group(P<0.01). The apoptosis rate of retinal cells of model group and intervention group was higher than that of the control group(P<0.01), that in the intervention group was lower than that in the model group(P<0.01). The expression of VEGF and GFAP in retina tissue of model group and intervention group was higher than that of the control group(P<0.01), and the expression of GLAST was lower than that in the control group(P<0.01). The expression of VEGF and GFAP in the intervention group was lower than that in the model group(P<0.01), and the expression of GLAST was higher than that in the model group(P<0.01).

CONCLUSION: Rhodiola sachalinensis injection can significantly improve the ocular blood flow in diabetic retinopathy rats, reduce the apoptosis of retinal cells in rats, down regulate the expression of VEGF and GFAP, and up regulate the expression of GLAST.